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城市空气中颗粒物在肺上皮细胞中产生活性氧的变化——无机成分的影响

Variations in Reactive Oxygen Species Generation by Urban Airborne Particulate Matter in Lung Epithelial Cells-Impact of Inorganic Fraction.

作者信息

Mazuryk Olga, Stochel Grazyna, Brindell Małgorzata

机构信息

Faculty of Chemistry, Jagiellonian University, Kraków, Poland.

出版信息

Front Chem. 2020 Dec 17;8:581752. doi: 10.3389/fchem.2020.581752. eCollection 2020.

DOI:10.3389/fchem.2020.581752
PMID:33392147
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7773840/
Abstract

Air pollution is associated with numerous negative effects on human health. The toxicity of organic components of air pollution is well-recognized, while the impact of their inorganic counterparts in the overall toxicity is still a matter of various discussions. The influence of airborne particulate matter (PM) and their inorganic components on biological function of human alveolar-like epithelial cells (A549) was investigated . A novel treatment protocol based on covering culture plates with PM allowed increasing the studied pollutant concentrations and prolonging their incubation time without cell exposure on physical suffocation and mechanical disturbance. PM decreased the viability of A549 cells and disrupted their mitochondrial membrane potential and calcium homeostasis. For the first time, the difference in the reactive oxygen species (ROS) profiles generated by organic and inorganic counterparts of PM was shown. Singlet oxygen generation was observed only after treatment of cells with inorganic fraction of PM, while hydrogen peroxide, hydroxyl radical, and superoxide anion radical were induced after exposure of A549 cells to both PM and their inorganic fraction.

摘要

空气污染对人类健康有诸多负面影响。空气污染中有机成分的毒性已得到充分认识，而其无机成分在整体毒性中的影响仍存在各种争议。研究了空气中颗粒物（PM）及其无机成分对人肺泡样上皮细胞（A549）生物学功能的影响。一种基于用PM覆盖培养板的新型处理方案，能够在不使细胞遭受物理窒息和机械干扰的情况下，提高所研究污染物的浓度并延长其孵育时间。PM降低了A549细胞的活力，破坏了其线粒体膜电位和钙稳态。首次显示了PM的有机和无机成分产生的活性氧（ROS）谱的差异。仅在用PM的无机部分处理细胞后才观察到单线态氧的产生，而在A549细胞暴露于PM及其无机部分后均诱导产生过氧化氢、羟基自由基和超氧阴离子自由基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/a4db0aa3ad7e/fchem-08-581752-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/39955e89976e/fchem-08-581752-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/0d1f1fe59e56/fchem-08-581752-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/aee4a08ce6ac/fchem-08-581752-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/7911fca5671a/fchem-08-581752-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/22be0847b8b5/fchem-08-581752-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/ce2a04ff9a1b/fchem-08-581752-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/0aaa2361b7a4/fchem-08-581752-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/a4db0aa3ad7e/fchem-08-581752-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/39955e89976e/fchem-08-581752-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/0d1f1fe59e56/fchem-08-581752-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/aee4a08ce6ac/fchem-08-581752-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/7911fca5671a/fchem-08-581752-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/22be0847b8b5/fchem-08-581752-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/ce2a04ff9a1b/fchem-08-581752-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/0aaa2361b7a4/fchem-08-581752-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f643/7773840/a4db0aa3ad7e/fchem-08-581752-g0007.jpg

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