Walker E J, Jeffrey P D, Webb J, Tymms K E
Protein Chemistry Group, John Curtin School of Medical Research, Australian National University, Canberra.
Clin Exp Rheumatol. 1989 Sep-Oct;7(5):537-40.
Anti-PL7 antibodies to threonyl-tRNA synthetase purified from beef liver were observed in the serum of a dermatopolymyositis patient. They were identified by CIE and DID using a standard anti-PL7 antiserum and quantified by ELISA in serum samples taken over a five year period. The level of antibodies against not only threonyl-tRNA synthetase but also several muscle proteins including tropomyosin, was strongly correlated with disease activity and treatment. This correlation may prove important both in monitoring the response of the patient to treatment and to our understanding of the aetiology of the disease.
在一名皮肌炎患者的血清中观察到了从牛肝中纯化出的抗苏氨酰 - tRNA合成酶的抗PL7抗体。使用标准抗PL7抗血清通过对流免疫电泳（CIE）和双免疫扩散（DID）对其进行鉴定，并通过酶联免疫吸附测定（ELISA）对在五年期间采集的血清样本进行定量。不仅针对苏氨酰 - tRNA合成酶，而且针对包括原肌球蛋白在内的几种肌肉蛋白的抗体水平与疾病活动和治疗密切相关。这种相关性在监测患者对治疗的反应以及我们对疾病病因的理解方面可能都具有重要意义。
