Targoff I N, Arnett F C, Reichlin M
Department of Medicine, Oklahoma University Health Science Center, Oklahoma City.
Arthritis Rheum. 1988 Apr;31(4):515-24. doi: 10.1002/art.1780310408.
The prevalence and clinical correlations of anti-threonyl-transfer RNA synthetase (anti-PL-7), as well as the relationship of anti-PL-7 to anti-histidyl-transfer RNA synthetase (anti-Jo-1) were studied in 109 sera from patients with myositis. Inhibition of threonine aminoacylation was used to screen for anti-PL-7. Sera from 3 patients, 2 with polymyositis and 1 with polymyositis-overlap syndrome, and a fourth serum from a patient with dermatomyositis, which was previously found to contain anti-PL-7, inhibited greater than 90% of activity (3.7% of 109 sera). All 4 sera reacted strongly in an enzyme-linked immunosorbent assay with enzyme that was either affinity purified with anti-PL-7 or was biochemically purified. There was no indication of cross-reactivity by aminoacylation inhibition or, for most sera, by enzyme-linked immunosorbent assay. Anti-PL-7 is an uncommon myositis-associated antibody that is independent of anti-Jo-1, but is directed at a functionally related enzyme.
在109例肌炎患者的血清中研究了抗苏氨酰转运RNA合成酶（抗PL - 7）的患病率及临床相关性，以及抗PL - 7与抗组氨酰转运RNA合成酶（抗Jo - 1）的关系。采用苏氨酸氨基酰化抑制法筛选抗PL - 7。3例患者的血清（2例多发性肌炎患者和1例多发性肌炎重叠综合征患者）以及1例皮肌炎患者的血清（该血清先前已发现含有抗PL - 7）抑制活性超过90%（109例血清中的3.7%）。所有这4例血清在酶联免疫吸附试验中与用抗PL - 7亲和纯化或生化纯化的酶均发生强烈反应。通过氨基酰化抑制试验，或者对于大多数血清而言通过酶联免疫吸附试验，均未显示交叉反应。抗PL - 7是一种罕见的与肌炎相关的抗体，它独立于抗Jo - 1，但针对一种功能相关的酶。
