Park In-Su, Chung Phil-Sang, Ahn Jin Chul
Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, Republic of Korea.
Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, Republic of Korea; Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, Republic of Korea.
Biochem Biophys Res Commun. 2015 Jul 3;462(3):171-7. doi: 10.1016/j.bbrc.2015.04.059. Epub 2015 Apr 21.
Human adipose-derived mesenchymal stem cells (hASCs) are attractive cell source for skin tissue engineering. The aim of this study was to investigate the effects of low-level light therapy (LLLT) on transplanted cluster hASC in a skin wound animal model. The hASCs were cultured in monolayer or clusters. The LLLT, hASCs, hASC clusters, and hASC clusters transplantation with LLLT (cluster + LLLT) were applied to the wound bed in athymic mice. Wound healing was assessed by gross evaluation and by hematoxylin and eosin staining, and elastin van gieson histochemistry. The survival, differentiation, and secretion of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF), and hepatocyte growth factor (HGF) of the cluster ASC were evaluated by immunohistochemistry and Western blotting. The cluster + LLLT group enhanced the wound healing, including neovascularization and regeneration of skin appendages, compared with the cluster group. The secretion of growth factors was stimulated in the cluster + LLLT group compared with the ASCs and cluster group. These data suggest that LLLT is an effective biostimulator of cluster hASCs in wound healing that enhances the survival of hASCs and stimulates the secretion of growth factors in the wound bed.
人脂肪间充质干细胞（hASCs）是皮肤组织工程中具有吸引力的细胞来源。本研究的目的是在皮肤创伤动物模型中研究低水平光疗（LLLT）对移植的成簇hASC的影响。hASCs以单层或成簇的方式培养。将LLLT、hASCs、hASC簇以及经LLLT处理的hASC簇移植（簇+LLLT）应用于无胸腺小鼠的伤口床。通过大体评估、苏木精-伊红染色以及弹性蛋白-凡吉森组织化学来评估伤口愈合情况。通过免疫组织化学和蛋白质印迹法评估簇状ASC的存活、分化以及血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（FGF）和肝细胞生长因子（HGF）的分泌情况。与簇状组相比，簇+LLLT组促进了伤口愈合，包括新生血管形成和皮肤附属器的再生。与ASC组和簇状组相比，簇+LLLT组中生长因子的分泌受到刺激。这些数据表明，LLLT是伤口愈合过程中簇状hASCs的一种有效生物刺激剂，可提高hASCs的存活率并刺激伤口床中生长因子的分泌。
