采用高效液相色谱-二极管阵列检测-质谱法测定葡萄牙东北部毒蝇伞蘑菇中的鹅膏毒素和鬼笔毒素。

Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS.

作者信息

Garcia Juliana, Oliveira Ana, de Pinho Paula Guedes, Freitas Victor, Carvalho Alexandra, Baptista Paula, Pereira Eric, de Lourdes Bastos Maria, Carvalho Félix

机构信息

Division of Medical Sciences & Graduate Entry Medicine, School of Medicine, Faculty of Medicine & Health Sciences, University of Nottingham, Royal Derby Hospital Centre, Derby, NG7 2UH, United Kingdom

出版信息

Mycologia. 2015 Jul-Aug;107(4):679-87. doi: 10.3852/14-253. Epub 2015 Apr 24.

DOI:10.3852/14-253

PMID:25911698

Abstract

Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. α-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.

摘要

毒鹅膏是一种有毒蘑菇，是蘑菇摄入后导致大多数死亡事件的罪魁祸首，主要原因是鹅膏毒素。在本研究中，采用液相色谱（LC）结合二极管阵列（DAD）和质谱（MS）检测技术，分析了来自葡萄牙两个不同地点的毒鹅膏不同组织中主要鹅膏毒素和鬼笔毒素的含量及分布。主要毒素通过液相色谱分离，其化学结构通过质谱确认。通过反相高效液相色谱（RP-HPLC）对菌盖、菌柄和菌托组织中的α-鹅膏毒肽含量进行了定量。结果表明，菌盖中鹅膏毒素含量最高，而菌托中鬼笔毒素含量最丰富。此外，还观察到来自不同地理位置的毒素组成存在差异。本研究首次提供了葡萄牙毒鹅膏中的毒素含量。