Enjalbert F, Gallion C, Jehl F, Monteil H, Faulstich H
Laboratoire de Botanique, Phytochimie et Mycologie, Faculté de Pharmacie, Montpellier, France.
J Chromatogr. 1992 May 15;598(2):227-36. doi: 10.1016/0021-9673(92)85052-u.
A reversed-phase high-performance liquid chromatographic method is described that allows the simultaneous determination of up to eight amatoxins and phallotoxins. The method identifies both neutral toxins (alpha- and gamma-amanitin, phalloidin, phallisin and phalloin) and acidic toxins (beta-amanitin, phallacidin and phallisacin). Toxins were separated, identified and determined by gradient elution with 0.02 M aqueous ammonium acetate-acetonitrile and simultaneous monitoring of the absorbances at 214 and 295 nm. The assay was successfully applied to the analysis of the toxins in a crude extract of Amanita phalloides. The limit of detection for each toxin was 10 ng/ml of extraction medium. The assay was further validated by analysing the toxin content in Galerina marginata, a species containing only amatoxins. This relatively simple method should be suitable for the detection of amatoxins and phallotoxins in almost any species of mushrooms.
本文描述了一种反相高效液相色谱法，该方法可同时测定多达八种鹅膏毒素和鬼笔毒素。该方法可鉴定中性毒素（α-和γ-鹅膏蕈碱、鬼笔环肽、鬼笔菌素和鬼笔原毒素）和酸性毒素（β-鹅膏蕈碱、鬼笔酸和鬼笔辛毒素）。通过用0.02 M乙酸铵水溶液-乙腈进行梯度洗脱，并同时监测214和295 nm处的吸光度，对毒素进行分离、鉴定和测定。该方法成功应用于毒鹅膏粗提物中毒素的分析。每种毒素的检测限为提取介质中10 ng/ml。通过分析仅含鹅膏毒素的环带盔孢伞中的毒素含量，进一步验证了该方法。这种相对简单的方法应适用于几乎任何种类蘑菇中鹅膏毒素和鬼笔毒素的检测。
