Liu Bo, Zhang Xiao-Ming, Guo Qiao-Sheng, Wang Chang-Lin, Chen Lu, Song Ling-Shan, An Kai-Long
Zhongguo Zhong Yao Za Zhi. 2014 Dec;39(23):4544-7.
To separate and identify chemical signals which induce Thesium chinense haustorium formation, the components of T. chinense roots secretion collected with XAD-4 resin were detected by GC-MS. The effect of DMBQ as exogenous signals to induce haustorium formation in T. chinense was studied. Fifty-three compounds of 9 types had been detected, including hydrocarbons, esters, organic acids, ketones, alcohols, nitrogen containing compounds, phenolic acids, aldehyde and quinine. It is worth noting that the 2, 5-di-tert-butyl-1,4-benzoquinone has the core structure of 1,4-benzoquinone, which may play an important role in the parasitic relationship of Prunella vulgaris and T. chinense: DMBQ worked effectively on inducing haustoria, but induction effects vary widely in different concentrations. DMBQ with the concentration of 1 μmol x L(-1) showed the best effect of the inducing ability with a ratio of 110.52 when treated to induce haustoria.
为了分离和鉴定诱导百蕊草吸器形成的化学信号，采用气相色谱-质谱联用仪（GC-MS）检测用XAD-4树脂收集的百蕊草根系分泌物的成分。研究了2,5-二叔丁基对苯二酚（DMBQ）作为外源信号诱导百蕊草吸器形成的作用。共检测到9类53种化合物，包括烃类、酯类、有机酸类、酮类、醇类、含氮化合物、酚酸类、醛类和醌类。值得注意的是，2,5-二叔丁基对苯二酚具有1,4-苯醌的核心结构，这可能在夏枯草与百蕊草的寄生关系中起重要作用：DMBQ对诱导吸器形成有效，但在不同浓度下诱导效果差异很大。当用浓度为1 μmol·L⁻¹的DMBQ处理诱导吸器时，诱导能力最佳，诱导率为110.52。
