Xiong Gengyan, Liu Jin, Tang Qi, Fan Yu, Fang Dong, Yang Kaiwei, Xie Feng, Zhang Min, Zhang Lei, Liu Libo, Zhang Cuijian, Yao Lin, Yang Li, Ci Weimin, Zhao Wei, Gong Yanqing, He Qun, Gong Kan, He Zhisong, Wang Gang, Li Xuesong, Guo Yinglu, Zhou Liqun
Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, National Urological Cancer Center, Beijing 100034, China.
Institute of Nephrology & Division of Nephrology, Peking University First Hospital, Beijing 100034, China.
Epigenomics. 2015 Aug;7(5):733-44. doi: 10.2217/epi.15.34. Epub 2015 Apr 27.
We conducted this study to identify gene promoter methylation status and clinical predictors for upper tract urothelial carcinoma (UTUC) patients.
MATERIALS & METHODS: Using methylation-sensitive PCR, we examined ten genes promoter methylation status in 687 UTUC patients.
A methylated promoter of three genes to predict higher tumor stage (T3 and T4), five genes to predict higher tumor grade (G3) and one gene to predict pN+ were certified in this study. Nine factors were significantly associated with poor cancer-specific survival. Six factors were considered as predictors to develop bladder recurrence after surgery.
Methylation occurs commonly in UTUCs, may affect carcinogenic mechanisms, and is a well predictive factor for cancer-specific survival and bladder recurrence in UTUCs.
我们开展本研究以确定上尿路尿路上皮癌（UTUC）患者的基因启动子甲基化状态及临床预测指标。
我们采用甲基化敏感PCR检测了687例UTUC患者中10个基因启动子的甲基化状态。
本研究证实，三个基因的甲基化启动子可预测更高的肿瘤分期（T3和T4），五个基因的甲基化启动子可预测更高的肿瘤分级（G3），一个基因的甲基化启动子可预测pN+。九个因素与较差的癌症特异性生存率显著相关。六个因素被视为术后发生膀胱复发的预测指标。
甲基化在UTUC中普遍存在，可能影响致癌机制，并且是UTUC患者癌症特异性生存和膀胱复发的良好预测因素。
