Soria Mario Alberto, Bonnet María Agustina, Bueno Dante Javier
Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Concepción del Uruguay, Casilla de Correo N° 6, 3260 Entre Ríos, Argentina.
Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Concepción del Uruguay, Casilla de Correo N° 6, 3260 Entre Ríos, Argentina.
Vet Immunol Immunopathol. 2015 Jun 15;165(3-4):145-53. doi: 10.1016/j.vetimm.2015.03.008. Epub 2015 Apr 6.
There are few studies about the blood serum of laying hens infected with Salmonella. The differential leukocyte count and blood chemistry values are an important aid in the diagnosis of human diseases, but blood parameters in the avian species are not well known. On the other hand, invasive forms of bacterial gastroenteritis, like Salmonella, often cause intestinal inflammation so this study was undertaken to find a biomarker of Salmonella infection and inflammatory intestinal response in the hematological or serum biochemical parameters in laying hens. Furthermore, we evaluated the association of some farm characteristics with Salmonella infection and fecal leukocytes (FL). A fecal sample with at least one fecal leukocyte per field was considered positive for inflammatory intestinal response. False positive serum reactions for Salmonella infection, by serum plate agglutination (SPA) test, were reduced by heating the sample to 56°C for 30 min and then diluting it 5-fold. The range of hematological and biochemical parameter values was very wide, in addition, there was a poor agreement between the SPA and FL results. Comparison of the positive and negative samples in SPA and FL showed that 1.3% and 79.8% of the laying hens were positive and negative in both tests, respectively. Hens with a positive SPA result showed a higher percentage of monocytes than those with a negative SPA result. Hens with a positive FL test had a higher percentage of heterophils, ratio of heterophils to lymphocytes and aspartate aminotransferase values, while the percentage of lymphocytes was significantly lower (P < 0.05) than those with a negative FL test. The risk of Salmonella infection increased when the age of laying hens and the number of hens per poultry house was greater than or equal to 18 months old and 10,000 laying hens, compared to less than 18 months old and 10,000 laying hens, respectively. On the other hand, the risk of inflammatory intestinal response was higher in laying hens ≥ 18 months old than in hens < 18 months old. Despite the fact that we did not find any specific biomarker of Salmonella infection, this is the first report about the change of Salmonella infection and inflammatory response in hematological/serum biochemical values for laying hens.
关于感染沙门氏菌的蛋鸡血清的研究很少。白细胞分类计数和血液生化值对人类疾病的诊断有重要帮助，但禽类的血液参数尚不为人所知。另一方面，侵袭性细菌性肠胃炎，如沙门氏菌感染，常引发肠道炎症，因此本研究旨在寻找蛋鸡血液学或血清生化参数中沙门氏菌感染及肠道炎症反应的生物标志物。此外，我们评估了一些养殖场特征与沙门氏菌感染及粪便白细胞（FL）之间的关联。每视野至少有一个粪便白细胞的粪便样本被视为肠道炎症反应呈阳性。通过血清平板凝集试验（SPA）检测沙门氏菌感染时，将样本加热至56°C 30分钟，然后5倍稀释，可减少血清反应假阳性。血液学和生化参数值范围很广，此外，SPA和FL结果之间的一致性较差。SPA和FL中阳性和阴性样本的比较显示，分别有1.3%和79.8%的蛋鸡在两项检测中呈阳性和阴性。SPA结果为阳性的母鸡单核细胞百分比高于SPA结果为阴性的母鸡。FL检测呈阳性的母鸡嗜异性粒细胞百分比、嗜异性粒细胞与淋巴细胞的比例以及天冬氨酸转氨酶值较高，而淋巴细胞百分比显著低于（P<0.05）FL检测呈阴性的母鸡。与分别小于18月龄和10000只蛋鸡相比，当蛋鸡年龄大于或等于1
