Kalyanasundaram Aravindan, Jawahar Shabnam, Ilangopathy Manikkavasagan, Palavesam Azahahianambi, Raman Muthusamy
Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India.
Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India; Translational Research Platform for Veterinary Biologicals (TRPVB), TANUVAS, II Floor, Central University Laboratory Building, Madhavaram Milk Colony, Chennai 600051, India.
Exp Parasitol. 2015 Jul;154:98-107. doi: 10.1016/j.exppara.2015.04.016. Epub 2015 Apr 23.
Haemonchus contortus is the most economically important blood feeding nematode parasite of sheep and goats all over the world. Enolase in helminth parasites is a multi-functional enzyme which involves in glycolysis and host tissue invasion. In this study, the recombinant H. contortus enolase (rHcENO) was evaluated for its immunoprophylactic efficacy in sheep along with Con A purified native glycoproteins in a vaccine challenge trial. Group I and Group II experimental sheep were immunized thrice with rHcENO and Con A purified native glycoproteins along with Montanide ISA 61 VG adjuvant. The animals were challenged with 5000 L3 stage active H. contortus larvae after 21 days of third immunization. A significant increase in the IgG titre was observed in rHcENO and Con A purified native glycoproteins immunized animals as compared to the control animals. Immunoprotective efficacy of Con A purified native glycoproteins was comparatively higher than rHcENO antigen.
捻转血矛线虫是全球对绵羊和山羊经济影响最为重大的吸血性线虫寄生虫。蠕虫寄生虫中的烯醇化酶是一种多功能酶，参与糖酵解和宿主组织入侵。在本研究中，在疫苗攻毒试验中评估了重组捻转血矛线虫烯醇化酶（rHcENO）与伴刀豆球蛋白A纯化的天然糖蛋白对绵羊的免疫预防效果。第一组和第二组实验绵羊用rHcENO和伴刀豆球蛋白A纯化的天然糖蛋白以及Montanide ISA 61 VG佐剂进行三次免疫。在第三次免疫21天后，用5000条L3期活性捻转血矛线虫幼虫对动物进行攻毒。与对照动物相比，在rHcENO和伴刀豆球蛋白A纯化的天然糖蛋白免疫的动物中观察到IgG滴度显著增加。伴刀豆球蛋白A纯化的天然糖蛋白的免疫保护效果相对高于rHcENO抗原。
