Davids Benjamin I, Davidson Megan J, TenBroeck Saundra H, Colahan Patrick T, Oli Monika W
Department of Animal Sciences, University of Florida, Gainesville, Florida.
College of Veterinary Medicine, University of Florida, Gainesville, Florida.
Vet Surg. 2015 Jul;44(5):648-52. doi: 10.1111/vsu.12335. Epub 2015 Apr 24.
To compare the efficacy of sterile preoperative skin antisepsis using either a 5-minute mechanical preparation or 5-minute non-mechanical preparation with chlorhexidine gluconate 4% solution.
Matched design, ANOVA.
Healthy adult Thoroughbred horses (n = 30).
Each horse had both surgical preparation methods randomly assigned to identical sites on the left or right upper thigh. Prepared sites were sampled and cultured for bacteria after each preparation step.
Mechanical and non-mechanical preparation techniques significantly reduced bacteria isolated from surface swab samples compared with samples taken from unprepared skin and after the preliminary rough prepared skin (P < 0.05). No difference in the number of skin-associated bacteria was detected between the mechanical and non-mechanical sterile preoperative preparation techniques (P = 0.77). Ten species of bacteria were identified by 16s PCR after final skin preparation.
Pre-surgical skin preparation without repeated mechanical scrubbing using chlorhexidine gluconate 4% solution (total contact time, 225 seconds) is effective in reducing bacterial counts.
比较使用4%葡萄糖酸氯己定溶液进行5分钟机械性术前皮肤消毒或5分钟非机械性术前皮肤消毒的效果。
配对设计，方差分析。
健康成年纯种马（n = 30）。
每匹马的两种手术准备方法随机分配至左右大腿上部相同部位。每个准备步骤后，对准备部位进行采样并培养细菌。
与未准备皮肤及初步粗糙准备皮肤后的样本相比，机械性和非机械性准备技术显著减少了从表面拭子样本中分离出的细菌（P < 0.05）。机械性和非机械性术前无菌准备技术之间未检测到皮肤相关细菌数量的差异（P = 0.77）。最终皮肤准备后，通过16s PCR鉴定出10种细菌。
使用4%葡萄糖酸氯己定溶液（总接触时间225秒）进行术前皮肤准备，无需反复机械擦洗，可有效减少细菌数量。
