Duffy P, Wallach J M
Laboratoire de Biochimie Analytique, UA CNRS244, Université de Lyon, Villeurbanne, France.
Enzyme. 1989;42(2):98-102. doi: 10.1159/000469015.
Serum cholinesterase activity was determined by conductimetry using samples in the microliter range. Butyrylcholine iodide was demonstrated to be a convenient substrate for the conductimetric assay. Validation of the microassay was made by using either purified enzyme or control serum. In the range of 0-60 U/l, a linear relationship was demonstrated. Correlation with a reference spectrophotometric method was obtained with a slope of 1.18. An explanation of this value is proposed, as different hydrolysis rates were obtained with human sera, depending on the substrate used (butyrylthio- or butyryl-choline ester).
采用微量范围的样本通过电导法测定血清胆碱酯酶活性。已证明碘化丁酰胆碱是电导测定法的便利底物。通过使用纯化酶或对照血清对微量测定法进行验证。在0 - 60 U/l范围内，呈现线性关系。与参考分光光度法的相关性斜率为1.18。针对该值给出了解释，因为根据所使用的底物（丁酰硫代胆碱或丁酰胆碱酯）不同，人血清获得的水解速率也不同。
