丁酰胆碱酯酶活性的微电导测定法。
Microconductimetric assay of butyrylcholinesterase activity.
作者信息
Duffy P, Wallach J M
出版信息
Biotechnol Appl Biochem. 1986 Oct;8(5):479-83.
PMID:3768149
Abstract
A conductimetric method for the assay of butyrylcholinesterase is described. A new conductimetric cell has been used which allows increased sensitivity. The physicochemical parameters (pH, buffer concentration) have been optimized. With 1 mM butyrylcholine, butyrylcholinesterase activities down to 2 x 10(-4) U ml-1 may be measured. Serum volumes needed for this assay are in the microliter range (1-5 microliters).
摘要
描述了一种用于测定丁酰胆碱酯酶的电导法。使用了一种新型电导池，可提高灵敏度。对物理化学参数（pH、缓冲液浓度）进行了优化。对于1 mM丁酰胆碱，可测量低至2×10⁻⁴ U ml⁻¹的丁酰胆碱酯酶活性。该测定所需的血清体积在微升范围内（1 - 5微升）。
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