Lv Jianjian, Zhang Dening, Gao Baoquan, Liu Ping, Li Jian
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071 Qingdao, China.
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071 Qingdao, China; College of Fisheries and Life Science, Shanghai Ocean University, 201306 Shanghai, China.
Gene. 2015 Jul 25;566(2):229-35. doi: 10.1016/j.gene.2015.04.050. Epub 2015 Apr 22.
Portunus trituberculatus is an important farmed crustacean in China. Growth traits are important economic traits that are controlled by many genes. The aim of this study was to screen growth-related genes using Illumina paired-end sequencing technology, and to identify growth-related SNPs in P. trituberculatus by association analysis. A total of 36,599,712 and 29,247,160 qualified Illumina read pairs for small size (SG) and large size crabs (LG) were obtained, giving rise to total clean bases of 7.14 and 5.72 G, respectively. A gene expression comparison showed that a total of 117 unigenes were differentially expressed between SG and LG, including 49 up-regulated and 68 down-regulated unigenes in SG and LG, respectively. GO functional enrichment analysis results showed up-regulated genes in LG were involved in three biological processes. These were cell communication, ovarian follicle cell development and cell division. Down-regulated genes in LG were involved in five biological processes, which were chitin metabolism, carbohydrate metabolism, ATP hydrolysis coupled proton transport, the steroid hormone mediated signaling pathway and fatty acid biosynthesis. Association analysis identified one growth-associated SNP, which was located in hemocyanin (comp58070-R31). This research has provided information on key genes, SNPs and biological processes related to growth, which will increase the efficiency of selective breeding programs for this important species.
三疣梭子蟹是中国重要的养殖甲壳类动物。生长性状是受许多基因控制的重要经济性状。本研究的目的是利用Illumina双末端测序技术筛选与生长相关的基因，并通过关联分析鉴定三疣梭子蟹中与生长相关的单核苷酸多态性（SNP）。分别从小体型（SG）和大体型蟹（LG）中获得了36,599,712对和29,247,160对合格的Illumina读段，产生的总净碱基分别为7.14 G和5.72 G。基因表达比较显示，SG和LG之间共有117个单基因差异表达，其中SG中有49个上调单基因，LG中有68个下调单基因。GO功能富集分析结果表明，LG中的上调基因参与了三个生物学过程。它们是细胞通讯、卵泡细胞发育和细胞分裂。LG中的下调基因参与了五个生物学过程，即几丁质代谢、碳水化合物代谢、ATP水解偶联质子转运、类固醇激素介导的信号通路和脂肪酸生物合成。关联分析鉴定出一个与生长相关的SNP，位于血蓝蛋白（comp58070-R31）中。本研究提供了与生长相关的关键基因、SNP和生物学过程的信息，这将提高该重要物种的选择性育种计划的效率。
