Key Laboratory for Sustainable Utilizatiyhuon of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Ministry of Agriculture and Rural, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao, 266003, People's Republic of China.
Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Mol Biol Rep. 2019 Feb;46(1):705-718. doi: 10.1007/s11033-018-4526-8. Epub 2018 Dec 1.
Growth is among the most important traits for animal breeding. Muscle growth is controlled by different cellular and molecular pathways and environments, and it also relies heavily on high-quality muscle contractions. The troponin complex, composed of troponin T (TnT), troponin C (TnC) and troponin I (TnI), plays a vital role in the regulation of muscle contraction. In this study, the cDNA of EcTnT, EcTnC and EcTnI of the ridgetail white prawn Exopalaemon carinicauda were cloned and characterized. The full length cDNA of EcTnT, EcTnC and EcTnI were 1 373 bp, 692 bp, and 1 475 bp, encoding a protein of 385, 150 and 193 amino acid residues, respectively. The expression of all genes was predominantly detected in abdominal muscle, while extremely lesser expressed in gill and hepatopancreas. Higher expression level of EcTnI was observed in heavier shrimp of the same age during different developmental stages, excepted for 120 days. Eleven single nucleotide polymorphisms (SNPs) were revealed in the three skeletal troponin genes, and only c.TnI66 A>G from EcTnI was significantly associated with both body weight and body length (P < 0.05). In summary, the result of this study suggested that EcTnI is growth-related gene of the troponin complex gene and the presence of SNP suggests that it could be a candidate gene for shrimp genetic improvement research.
生长是动物育种中最重要的特征之一。肌肉生长受不同的细胞和分子途径和环境控制，并且还严重依赖于高质量的肌肉收缩。肌钙蛋白复合物由肌钙蛋白 T（TnT）、肌钙蛋白 C（TnC）和肌钙蛋白 I（TnI）组成，在调节肌肉收缩中起着至关重要的作用。本研究克隆并鉴定了脊尾白虾 Exopalaemon carinicauda 的 EcTnT、EcTnC 和 EcTnI 的 cDNA。EcTnT、EcTnC 和 EcTnI 的全长 cDNA 分别为 1373bp、692bp 和 1475bp，编码 385、150 和 193 个氨基酸残基的蛋白质。所有基因的表达主要在腹部肌肉中检测到，而在鳃和肝胰腺中表达极少。在不同发育阶段，相同年龄的虾中 EcTnI 的表达水平较高，除了 120 天外。在三个骨骼肌钙蛋白基因中发现了 11 个单核苷酸多态性（SNP），只有 EcTnI 中的 c.TnI66 A>G 与体重和体长均显著相关（P<0.05）。综上所述，本研究结果表明 EcTnI 是肌钙蛋白复合物基因的生长相关基因，SNP 的存在表明它可能是虾遗传改良研究的候选基因。
