Kukusamude Chunyapuk, Srijaranai Supalax, Quirino Joselito P
Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Australia.
Materials Chemistry Research Unit, Department of Chemistry and Centre of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
J Sep Sci. 2015 Jul;38(14):2532-7. doi: 10.1002/jssc.201500268.
Interface-free two-dimensional heart-cutting capillary electrophoresis for two different classes of analytes (anionic and neutral) in a single capillary is presented. Simultaneous sample stacking and orthogonal separation were demonstrated. The anionic species were first analyzed by capillary zone electrophoresis in the first dimension. Then, the neutral compounds were separated in the second dimension by micellar electrokinetic chromatography using the common anionic surfactant sodium dodecyl sulfate. The first and second dimensions occurred automatically without changing the electrolyte and without polarity switching. Artificial mixtures (five anions and four neutral compounds) were successfully analyzed with sensitivity enhancement factors from 7 to 28. The orthogonal separation was complete within 8 min. Some analytical features and application to a spiked real river water sample were also studied.
本文介绍了一种无界面二维心切割毛细管电泳方法，可在单一毛细管中同时分析两类不同的分析物（阴离子和中性物质）。该方法实现了同时进样堆积和正交分离。首先在第一维通过毛细管区带电泳分析阴离子物质。然后，在第二维使用常见的阴离子表面活性剂十二烷基硫酸钠通过胶束电动色谱分离中性化合物。第一维和第二维的分离自动进行，无需更换电解质，也无需进行极性切换。人工混合物（五种阴离子和四种中性化合物）得到成功分析，灵敏度增强因子为7至28。8分钟内即可完成正交分离。还研究了该方法的一些分析特性及其在加标实际河水样品中的应用。
