Tavakoli Maryam, Salek-Moghaddam Alireza, Jeddi-Tehrani Mahmood, Talebi Saeed, Kazemi-Sefat Golnaz-Ensieh, Vafaei Sedigheh, Mohammadzadeh Afsaneh, Sheikhhassani Shahrzad, Zarnani Amir-Hassan
Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mol Reprod Dev. 2015 May;82(5):356-64. doi: 10.1002/mrd.22486. Epub 2015 Apr 23.
Vitamin D exerts important roles during pregnancy, and its deficiency may be associated with several pregnancy complications, including pregnancy loss, yet no data are available for molecules involved in vitamin D metabolism in patients with unexplained recurrent spontaneous abortion. In this study, we investigated possible difference in endometrial expression of vitamin D3 receptor (VDR), 1α-hydroxylase (CYP27B1), and 24-hydroxylase (CYP24A1) in women with recurrent spontaneous abortion (n = 8) and healthy controls (n = 8). Gene expression of VDR, CYP27B1, and CYP24A1 was determined by real-time PCR, while VDR and CYP27B1 proteins were localized by immunohistochemistry and their abundance was validated by Western blot. We found that both patient and control groups expressed comparable levels of endometrial VDR, CYP27B1, and CYP24A1 transcripts. In line with the gene-expression results, CYP27B1 and different isoforms of VDR protein were present at the same abundance in the endometria of both groups. No significant alteration in VDR and CYP27B1 immunoreactivity pattern was found in the endometrium of patients compared to fertile controls, however. The results of the present study, therefore, do not support the hypothesis of differential expression of key molecules involved in vitamin D3 metabolism in the endometrium of recurrent spontaneous abortion patients and fertile controls.
维生素D在孕期发挥着重要作用，其缺乏可能与多种妊娠并发症相关，包括流产，但目前尚无关于不明原因复发性自然流产患者维生素D代谢相关分子的数据。在本研究中，我们调查了复发性自然流产女性（n = 8）和健康对照者（n = 8）子宫内膜中维生素D3受体（VDR）、1α-羟化酶（CYP27B1）和24-羟化酶（CYP24A1）表达的可能差异。通过实时PCR测定VDR、CYP27B1和CYP24A1的基因表达，同时通过免疫组织化学对VDR和CYP27B1蛋白进行定位，并通过蛋白质印迹法验证其丰度。我们发现患者组和对照组子宫内膜VDR、CYP27B1和CYP24A1转录本的表达水平相当。与基因表达结果一致，两组子宫内膜中CYP27B1和不同亚型的VDR蛋白丰度相同。然而，与可育对照相比，患者子宫内膜中VDR和CYP27B1免疫反应模式没有明显改变。因此，本研究结果不支持复发性自然流产患者和可育对照者子宫内膜中维生素D3代谢关键分子差异表达的假说。
