Mellott Adam J, Shinogle Heather E, Moore David S, Detamore Michael S
Bioengineering Graduate Program, University of Kansas, Lawrence, Kansas 66045.
Microscopy and Analytical Imaging Laboratory, University of Kansas, Lawrence, Kansas 66045.
Cell Mol Bioeng. 2015 Mar 1;8(1):187-196. doi: 10.1007/s12195-014-0365-4.
Not all cells behave uniformly after treatment in tissue engineering studies. In fact, some treated cells display no signs of treatment or show unique characteristics not consistent with other treated cells. What if the "unique" cells could be isolated from a treated population, and further studied? Photo-convertible reporter proteins, such as , allow for the ability to selectively identify unique cells with a secondary label within a primary labeled treated population. In the current study, select cells were identified and labeled through photo-conversion of -transfected human Wharton's Jelly cells (hWJCs) for the first time. Robust photo-conversion of green-to-red fluorescence was achieved consistently in arbitrarily selected cells, allowing for precise cell identification of select hWJCs. The current study demonstrates a method that offers investigators the opportunity to selectively label and identify unique cells within a treated population for further study or isolation from the treatment population. Photo-convertible reporter proteins, such as , offer the ability over non-photo-convertible reporter proteins, such as green fluorescent protein, to analyze unique individual cells within a treated population, which allows investigators to gain more meaningful information on how a treatment affects all cells within a target population.
在组织工程研究中，并非所有细胞在处理后都表现一致。事实上，一些处理过的细胞没有显示出处理的迹象，或者表现出与其他处理过的细胞不一致的独特特征。如果能从处理后的细胞群体中分离出这些“独特”的细胞并进一步研究呢？光转化报告蛋白，如 ，能够在初次标记的处理细胞群体中，利用二次标记选择性地识别独特的细胞。在当前的研究中，首次通过对转染 的人脐带华通氏胶细胞（hWJCs）进行光转化来识别和标记特定细胞。在任意选择的细胞中均持续实现了从绿色荧光到红色荧光的强大光转化，从而能够精确识别特定的hWJCs。当前的研究展示了一种方法，为研究人员提供了机会，可在处理后的细胞群体中选择性地标记和识别独特的细胞，以便进一步研究或将其与处理群体分离。光转化报告蛋白，如 ，相对于非光转化报告蛋白，如绿色荧光蛋白，具有分析处理细胞群体中独特个体细胞的能力，这使研究人员能够获得更有意义的信息，了解处理如何影响目标群体中的所有细胞。
