Shahid Adangam Purath, Salini Sasidharan, Sasidharan Nanu, Padikkala Jose, Raghavamenon Achuthan Chathrattil, Babu Thekkekara Devassy
J Basic Clin Physiol Pharmacol. 2015 Sep;26(5):509-15. doi: 10.1515/jbcpp-2014-0124.
Estrogen-mediated uterus endometrium instability is considered as one of the etiological factors in dysfunctional uterine bleeding (DUB) and uterine cancer. Saraca asoca (Family: Fabaceae) and its fermented preparation, Asokarishta, are extensively used as uterine tonic to treat gynecological disorders in Ayurveda. The present study evaluated the effect of S. asoca (Asoka) on estrogen-induced endometrial thickening of rat uterus.
Endometrial thickening was induced by intraperitoneal injection of estradiol (20 μg/kg b.wt) to 8-day-old immature rats for alternate 5 days. Methanolic extract (200 mg/kg b. wt) from S. asoca bark was given orally along with estradiol. Uterus endometrial thickening was analyzed histopathologically and serum estrogen level by radioimmunoassay (RIA). Cyclooxygenase (COX-2) expression in rat uterus was also estimated by Western blot. Anti-inflammatory activity of the extract was analyzed by formalin- and carrageenan-elicited paw edema models in mouse.
Uterus endometrium proliferation and keratinized metaplasia with seven to eight stratified epithelial layers on day 16 was observed in rats administered with estradiol. Treatment with S. asoca reduced the thickening to two to four layers and the serum estrogen level diminished significantly to 82.9±12.87 pg/mL compared to rats administered with estrogen alone (111.2±10.68 pg/mL). A reduction of formalin- and carrageenan-induced paw edema in mouse by S. asoca extract was observed. Lower level of lipopolysaccharides (LPS)-induced COX-2 enzyme in rat uterus by the extract further confirms its anti-inflammatory activity.
Present study reveals the antiproliferative and antikeratinizing effects of S. asoca in uterus endometrium possibly through its anti-estrogenic and anti-inflammatory properties.
雌激素介导的子宫内膜不稳定被认为是功能性子宫出血（DUB）和子宫癌的病因之一。无忧花（豆科）及其发酵制剂阿索卡里斯塔，在阿育吠陀医学中被广泛用作子宫滋补剂来治疗妇科疾病。本研究评估了无忧花对雌激素诱导的大鼠子宫内膜增厚的影响。
对8日龄未成熟大鼠腹腔注射雌二醇（20μg/kg体重），隔日注射，共5天，以诱导子宫内膜增厚。将无忧花树皮的甲醇提取物（200mg/kg体重）与雌二醇一起口服。通过组织病理学分析子宫子宫内膜增厚情况，并通过放射免疫分析（RIA）测定血清雌激素水平。还通过蛋白质免疫印迹法估计大鼠子宫中环氧化酶（COX-2）的表达。通过小鼠福尔马林和角叉菜胶诱发的爪肿胀模型分析提取物的抗炎活性。
在给予雌二醇的大鼠中，第16天观察到子宫内膜增殖和角化化生，有七到八层分层上皮。与单独给予雌激素的大鼠（111.2±10.68pg/mL）相比，无忧花治疗使增厚减少至两到四层，血清雌激素水平显著降低至82.9±12.87pg/mL。观察到无忧花提取物可减轻小鼠福尔马林和角叉菜胶诱发的爪肿胀。提取物使大鼠子宫中脂多糖（LPS）诱导的COX-2酶水平降低，进一步证实了其抗炎活性。
本研究揭示了无忧花对子宫内膜可能具有抗增殖和抗角化作用，可能是通过其抗雌激素和抗炎特性实现的。
