编码小鼠Clq B链的互补DNA的分子克隆与特性分析
Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq.
作者信息
Petry F, Reid K B, Loos M
机构信息
Institute of Medical Microbiology, Johannes Gutenberg-University, Mainz, FRG.
出版信息
FEBS Lett. 1989 Nov 20;258(1):89-93. doi: 10.1016/0014-5793(89)81622-9.
cDNA clones coding for the B-chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B-chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate-activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues.
从小鼠巨噬细胞文库中分离出编码小鼠Clq B链的cDNA克隆。所鉴定的克隆包括完整的编码区加上一个前导序列。在编码区发现与人Clq B链具有高度同源性（81%）。对Balb/c小鼠不同组织的总RNA进行Northern印迹分析，显示出一条约1.2 kb的条带。在巯基乙酸激活的腹腔巨噬细胞的RNA制剂中发现最高信号。该探针也与脾脏、胸腺和心脏的mRNA杂交。在肝脏、肾脏、肺和肠道组织中发现极弱的信号。
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