编码补体C1q完整鼠源B链的cDNA克隆：核苷酸序列及推导的氨基酸序列

cDNA clones coding for the complete murine B chain of complement Clq: nucleotide and derived amino acid sequences.

作者信息

Wood L, Pulaski S, Vogeli G

机构信息

Molecular Biology Research 7242, Upjohn Company, Kalamazoo, Michigan 49001.

出版信息

Immunol Lett. 1988 Jan;17(1):59-62. doi: 10.1016/0165-2478(88)90102-2.

DOI:10.1016/0165-2478(88)90102-2

PMID:3258283

Abstract

We have isolated cDNA clones covering the complete B chain of the complement subunit Clq from mouse; this subunit initiates the classical complement pathway. Deoxynucleotide sequence analysis shows that these clones contain 156 nucleotides of the 5' untranslated region, followed by sequences coding for the 25 amino acids of the signal peptide; all of the 228 amino acids of the mature protein; and 140 nucleotides of the 3' untranslated region, including a poly A additional signal. The coding region for the mature protein contains 261 nucleotides for the Gly-X-Y repeat and 408 nucleotides for the globular portion of Clq. By comparing the nucleotide sequence of the mouse B chain of Clq with the human B chain (Reid, K.B.M., 1985, Biochem. J. 231, 729), we find a high homology (80%) within the mature protein, a lower homology within the signal peptide (59%) and the 3' untranslated region (47%) and no homology (26%) in the 5' untranslated region.

摘要

我们已从小鼠中分离出覆盖补体亚基C1q完整B链的cDNA克隆；该亚基启动经典补体途径。脱氧核苷酸序列分析表明，这些克隆包含5'非翻译区的156个核苷酸，接着是编码信号肽25个氨基酸的序列；成熟蛋白的全部228个氨基酸；以及3'非翻译区的140个核苷酸，包括一个多聚腺苷酸附加信号。成熟蛋白的编码区包含261个核苷酸用于Gly-X-Y重复序列，408个核苷酸用于C1q的球状部分。通过比较小鼠C1q B链与人B链的核苷酸序列（Reid，K.B.M.，1985，《生物化学杂志》231，729），我们发现在成熟蛋白内有高度同源性（80%），在信号肽内同源性较低（59%），在3'非翻译区内同源性更低（47%），而在5'非翻译区内无同源性（26%）。