Morel F, Vandenberghe Y, Pemble S, Taylor J B, Ratanasavanh D, Rogiers V, Ketterer B, Guillouzo A
INSERM U49, Hôpital de Pontchaillou, Rennes, France.
FEBS Lett. 1989 Nov 20;258(1):99-102. doi: 10.1016/0014-5793(89)81624-2.
mRNA levels of glutathione S-transferase (GST) subunits 3 and 4 were measured with a specific cDNA probe in adult rat hepatocytes maintained either in conventional culture or in coculture with rat liver epithelial cells. Four media conditions were used, i.e. with or without fetal calf serum (FCS) and with nicotinamide or dimethylsulfoxide (DMSO). When FCS was present in the culture medium, GST subunit 3 and 4 mRNAs were expressed at a level close to that found in freshly isolated hepatocytes during the whole culture period both in conventional culture and in coculture. All other culture conditions resulted in an increase of GST 3 and 4 mRNA levels. After exposure to phenobarbital an increase in GST 3 and 4 mRNA levels was demonstrated in both culture systems. Comparison with previous findings on the expression of GST subunits 1, 2 and 7 in the same culture conditions indicates that the different classes of GST are regulated independently.
使用特异性cDNA探针，在常规培养或与大鼠肝上皮细胞共培养的成年大鼠肝细胞中，测定谷胱甘肽S-转移酶（GST）亚基3和4的mRNA水平。采用了四种培养基条件，即添加或不添加胎牛血清（FCS），以及添加烟酰胺或二甲基亚砜（DMSO）。当培养基中存在FCS时，在整个培养期间，无论是常规培养还是共培养，GST亚基3和4的mRNA表达水平都接近新鲜分离的肝细胞中的水平。所有其他培养条件均导致GST 3和4的mRNA水平升高。在两种培养系统中，接触苯巴比妥后，GST 3和4的mRNA水平均升高。与之前在相同培养条件下关于GST亚基1、2和7表达的研究结果相比，表明不同类别的GST是独立调节的。
