Calvo L, Vantman D, Banks S M, Tezon J, Koukoulis G N, Dennison L, Sherins R J
Developmental Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, Maryland.
Fertil Steril. 1989 Dec;52(6):1048-54. doi: 10.1016/s0015-0282(16)53173-2.
We compared the ability of sperm to undergo follicular fluid-induced acrosome reaction in vitro in fertile men and patients with unexplained infertility. After capacitation under optimum conditions, 28% of sperm from fertile men undergo acrosome reaction after follicular fluid exposure, whereas only 7% of the cells react spontaneously. In 15 men with unexplained infertility, 6 patients showed lack of acrosome reaction, whereas 9 men had sperm acrosome reactions similar to that of fertile men. However, in this cohort under study, semen characteristics of AR(+) and AR(-) patients were similar. In addition to inducing sperm acrosome reaction, follicular fluid also promoted significant changes in motion characteristics of capacitated sperm. Sperm curvilinear velocity (Vc) increased significantly after exposure to follicular fluid though linearity remained unchanged. The largest difference in cumulative Vc occurred at 90 microns/s. Assessing the ability of capacitated sperm to acrosome react may have clinical significance in predicting whether such sperm are capable of fertilizing an ovum.
我们比较了正常生育男性和不明原因不孕症患者的精子在体外发生卵泡液诱导的顶体反应的能力。在最佳条件下进行获能后，正常生育男性的精子在暴露于卵泡液后有28%发生顶体反应，而只有7%的细胞自发反应。在15名不明原因不孕症男性中，6名患者表现出缺乏顶体反应，而9名男性的精子顶体反应与正常生育男性相似。然而，在本研究队列中，顶体反应阳性（AR(+)）和顶体反应阴性（AR(-)）患者的精液特征相似。除了诱导精子顶体反应外，卵泡液还促使获能精子的运动特征发生显著变化。暴露于卵泡液后，精子曲线速度（Vc）显著增加，尽管直线性保持不变。累积Vc的最大差异出现在90微米/秒。评估获能精子发生顶体反应的能力对于预测此类精子是否能够使卵子受精可能具有临床意义。
