Saenz-de-Juano Maria Desemparats, Vicente José Salvador, Hollung Kristin, Marco-Jiménez Francisco
Instituto de Ciencia y Tecnología Animal, Universidad Politècnica de València, Valencia, Spain.
Nofima AS, Aas, Norway.
PLoS One. 2015 Apr 27;10(4):e0125157. doi: 10.1371/journal.pone.0125157. eCollection 2015.
Very limited information on the post-implantatory effects of vitrification has been published till now. We observed in a previous study that the vitrification procedure for the cryopreservation of embryos introduced transcriptomic and proteomic modifications in the rabbit foetal placenta at the middle of gestation. Now, we have conducted a proteomic study to determine whether protein alterations in the foetal placenta induced by the vitrification procedure remain during pregnancy. In this study, we used 2D-DIGE and mass spectrometry (MALDI-TOF-TOF and LC-MS/MS analysis) to identify the protein changes during middle and late stages of gestation (Day 14 and Day 24, respectively) in rabbit foetal placenta. We identified 11 differentially expressed proteins at Day 14 and 13 proteins at Day 24. Data are available via ProteomeXchange with identifiers PXD001840 and PXD001836. In addition, we demonstrate the presence of three proteins, serum albumin, isocitrate dehydrogenase 1 [NADP+], and phosphoglycerate mutase 1, which were altered during pregnancy. We demonstrate the existence of changes in foetal placental protein during pregnancy induced by the vitrification procedure, which brings into question whether vitrification effects observed during foetal development could lead to physiological and metabolic disorders in adulthood. This effect, taken together with other effects reported in the literature, suggests that embryo cryopreservation is not neutral.
到目前为止，关于玻璃化冷冻术后影响的信息非常有限。我们在之前的一项研究中观察到，用于胚胎冷冻保存的玻璃化程序在妊娠中期对兔胎儿胎盘引入了转录组和蛋白质组的改变。现在，我们进行了一项蛋白质组学研究，以确定玻璃化程序诱导的胎儿胎盘蛋白质改变在妊娠期间是否持续存在。在这项研究中，我们使用二维差异凝胶电泳（2D-DIGE）和质谱（基质辅助激光解吸电离飞行时间质谱和液相色谱-串联质谱分析）来鉴定兔胎儿胎盘在妊娠中期和晚期（分别为第14天和第24天）的蛋白质变化。我们在第14天鉴定出11种差异表达蛋白质，在第24天鉴定出13种蛋白质。数据可通过蛋白质组交换库获取，标识符分别为PXD001840和PXD001836。此外，我们证明了三种蛋白质的存在，即血清白蛋白、异柠檬酸脱氢酶1[NADP+]和磷酸甘油酸变位酶1，它们在妊娠期间发生了改变。我们证明了玻璃化程序在妊娠期间诱导胎儿胎盘蛋白质发生变化，这引发了一个问题，即胎儿发育过程中观察到的玻璃化影响是否会导致成年期的生理和代谢紊乱。这种影响，连同文献中报道的其他影响，表明胚胎冷冻保存并非没有影响。
