Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
The Key Laboratory for Reproductive Medicine of Guangdong Province, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Gynecol Endocrinol. 2020 Nov;36(11):973-977. doi: 10.1080/09513590.2020.1734785. Epub 2020 Mar 4.
Vitrification is widely used in assisted reproductive technologies. However, the nervous system of vitrification offspring is of concern, and research on this is lacking. Vitrification-born mice (vitrification group), conventional fertilization-embryo transfer pregnancy-born mice (IVF group), and natural pregnancy-born mice (control group) were used to study the effects of vitrification of mouse embryos on protein levels in the brain of offspring. Proteins differentially expressed among the three groups were analyzed using proteomic methods, including two-dimensional electrophoresis, mass spectrometry, and bioinformatics analysis. Immunohistochemistry was used to verify the expression of differentially expressed proteins, such as Actb and Actg1, in each group. Twenty differentially expressed proteins in the brain tissue were identified using two-dimensional protein electrophoresis and mass spectrometry. Bioinformatics analysis revealed that these proteins were related to the development of anatomical structure, signal transduction, transport, cell differentiation, and stress response (biological processes) and the binding of molecules (molecular functions). The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the differentially expressed proteins were involved in 54 pathways, including phagosome, metabolic pathway, apoptosis, and cysteine and methionine metabolism. Thus, embryo vitrification may cause some changes in the mouse brain at the protein level, necessitating further safety assessment.
玻璃化技术广泛应用于辅助生殖技术。然而，玻璃化胚胎的神经系统令人担忧，相关研究却很少。本研究使用玻璃化出生的小鼠（玻璃化组）、常规体外受精-胚胎移植妊娠出生的小鼠（IVF 组）和自然妊娠出生的小鼠（对照组），研究胚胎玻璃化对后代大脑中蛋白质水平的影响。采用蛋白质组学方法，包括二维电泳、质谱和生物信息学分析，分析三组之间差异表达的蛋白质。免疫组织化学用于验证各组中差异表达蛋白（如 Actb 和 Actg1）的表达。通过二维蛋白质电泳和质谱鉴定出脑组织中 20 种差异表达蛋白。生物信息学分析表明，这些蛋白与解剖结构发育、信号转导、运输、细胞分化和应激反应（生物过程）以及分子结合（分子功能）有关。京都基因与基因组百科全书通路分析表明，差异表达蛋白涉及 54 条通路，包括吞噬体、代谢途径、细胞凋亡、半胱氨酸和蛋氨酸代谢。因此，胚胎玻璃化可能会导致小鼠大脑在蛋白质水平上发生一些变化，需要进一步进行安全性评估。
