Wang Dadong, Tan Jingwang, Xu Yong, Tan Xianglong, Han Mingming, Tu Yuliang, Zhu Ziman, Zen Jianping, Dou Chunqing, Cai Shouwang
Hepatogastroenterology. 2015 Mar-Apr;62(138):378-82.
The aim of the study is to identify the differentially expressed microRNAs (miRNAs) between hepatocellular carcinoma (HCC) samples and controls and provide new diagnostic potential miRNAs for HCC. The miRNAs expression profile data GSE20077 included 7 HCC samples, 1 HeLa sample and 3 controls. Differentially expressed miRNAs (DE-miRNAs) were identified by t-test and wilcox test. The miRNA with significantly differential expression was chosen for further analysis. Target genes for this miRNA were selected using TargetScan and miRbase database. STRING software was applied to construct the target genes interaction network and topology analysis was carried out to identify the hub gene in the network. And we identified the mechanism for affecting miRNA function. A total of 54 differentially expressed miRNAs were identified, in which there were 13 miRNAs published to be related to HCC. The differentially expressed hsa-miR-106b was chosen for further analysis and PTPRT (Receptor-type tyrosine-protein phosphatase T) was its potential target gene. The target genes interaction network was constructed among 33 genes, in which PTPRT was the hub gene. We got the conclusion that the differentially expressed hsa-miR-106b may play an important role in the development of HCC by regulating the expression of its potential target gene PT-PRT.
本研究的目的是鉴定肝细胞癌（HCC）样本与对照之间差异表达的微小RNA（miRNA），并为HCC提供新的具有诊断潜力的miRNA。miRNA表达谱数据GSE20077包括7个HCC样本、1个HeLa样本和3个对照。通过t检验和威尔科克森检验鉴定差异表达的miRNA（DE-miRNA）。选择具有显著差异表达的miRNA进行进一步分析。使用TargetScan和miRbase数据库选择该miRNA的靶基因。应用STRING软件构建靶基因相互作用网络，并进行拓扑分析以鉴定网络中的枢纽基因。并且我们确定了影响miRNA功能的机制。共鉴定出54个差异表达的miRNA，其中有13个miRNA已发表与HCC相关。选择差异表达的hsa-miR-106b进行进一步分析，PTPRT（受体型酪氨酸蛋白磷酸酶T）是其潜在靶基因。在33个基因之间构建了靶基因相互作用网络，其中PTPRT是枢纽基因。我们得出结论，差异表达的hsa-miR-106b可能通过调节其潜在靶基因PT-PRT的表达在HCC的发生发展中起重要作用。
