Department of Oncology, ShaoXing Municipal Hospital, Shaoxing, Zhejiang, P.R. China.
Department of Interventional Radiology, The 4th Hospital of Hebei Medical University, Shi Jiazhuang, Hebei, P.R. China.
Oncol Res. 2018 Sep 14;26(8):1183-1189. doi: 10.3727/096504018X15151495109394. Epub 2018 Jan 10.
Recent studies have revealed abnormal expression of miRNAs in various tumors. Although microRNA-338-3p (miR-338-3p) plays an important role in many types of tumors, its influence on liver cancer (LC) is unknown. In this study, we found that expression of miR-338-3p was decreased in LC cells and tissues. Colony formation and cell proliferation were suppressed by enhanced expression of miR-338-3p in LC cells. Moreover, miR-338-3p targeted sphingosine kinase 2 (SphK2). Silencing of SphK2 had an identical influence as overexpression of miR-338-3p in LC cells. Overexpression of SphK2 without the 3'-untranslated region remarkably enhanced the growth suppression triggered by miR-338-3p in LC cells. These findings indicate that miR-338-3p influences the development of LC by targeting SphK2, suggesting that miR-338-3p can be targeted as an innovative therapeutic strategy for LC.
最近的研究揭示了 miRNA 在各种肿瘤中的异常表达。虽然 microRNA-338-3p（miR-338-3p）在许多类型的肿瘤中发挥着重要作用，但它对肝癌（LC）的影响尚不清楚。在本研究中，我们发现 miR-338-3p 在 LC 细胞和组织中的表达降低。在 LC 细胞中增强 miR-338-3p 的表达会抑制集落形成和细胞增殖。此外，miR-338-3p 靶向鞘氨醇激酶 2（SphK2）。在 LC 细胞中沉默 SphK2 与过表达 miR-338-3p 具有相同的影响。没有 3'-非翻译区的 SphK2 的过表达显著增强了 miR-338-3p 在 LC 细胞中引发的生长抑制作用。这些发现表明 miR-338-3p 通过靶向 SphK2 影响 LC 的发展，表明 miR-338-3p 可以作为 LC 的一种创新治疗策略。
