Wang C, Feng B
Mol Biol (Mosk). 2015 Jan-Feb;49(1):3-25. doi: 10.7868/s0026898415010176.
In today's post-genome era, the goals of research are geared toward understanding the meaning of information in sequenced DNA, namely, understanding the functional characteristics of proteins encoded by genomes of living beings. Protein array technologies, particularly miniaturized high-throughput platforms such as micro- or nano-fluidic chips that allow the parallel detection of thousands of proteins simultaneously, are playing increasing important roles as discovery tools in proteomics. These technologies are based on principles of molecular recognition and consist of a support surface, such as a glass slide, bead, or microtiter plate, to which an array of captured proteins is bound. However, immobilized proteins often lose their immunoactivity and suffer from low surface density, which results in inefficient signal response. In this review, we mainly provide an introduction about the research progress on site-oriented adsorption of protein at solid-liquid interfaces, especially the three-dimensional immobilization of protein, whose objective is to retain immobilized proteins in an active state at great density.
在当今的后基因组时代，研究目标旨在理解测序DNA中信息的含义，即了解生物体基因组编码蛋白质的功能特性。蛋白质阵列技术，尤其是诸如微流控或纳流控芯片等小型化高通量平台，能够同时对数千种蛋白质进行平行检测，作为蛋白质组学中的发现工具正发挥着越来越重要的作用。这些技术基于分子识别原理，由诸如载玻片、珠子或微量滴定板等支持表面组成，一系列捕获的蛋白质结合在该支持表面上。然而，固定化蛋白质常常会失去其免疫活性，且表面密度较低，这导致信号响应效率低下。在本综述中，我们主要介绍了蛋白质在固液界面上的定点吸附，尤其是蛋白质的三维固定化的研究进展，其目的是将固定化蛋白质以高密度保持在活性状态。
