Zhdanov R I, Kern D, Lorents V, Ibragimova M Ia
Mikrobiologiia. 2015 Jan-Feb;84(1):50-7.
An approach used on investigation of the lipid composition of loosely (fraction 1) and tightly (fraction 2) DNA-bound lipids of Pseudomonas aurantiaca cells by electrospray ionization using mass spectrometry (ESI-LC-MS) was used for determination of the lipidom of a prokaryotic cell. Free fatty acids C16:0, C18:1 (fraction 1), C14:0, C16:0, and C18:2 (fraction 2) were detected. Both fractions of DNA-bound lipids were characterized by the presence of phosphatidylglycerol, phosphatidylserine, and lyso-phosphatidylinositol. The alcohol-soluble fraction 1 could also contain phosphatidylcholine and phosphatidylinositol, while fraction 2 probably contained triacylglycerides. Compared to gas chromatography, ESI-LC-MS provides new possibilities for investigation of the nucleoid lipidome, providing for more detailed investigation of DNA-bound lipids in bacterial cells.
采用电喷雾电离质谱法（ESI-LC-MS）对橙色假单胞菌细胞中与DNA松散结合（组分1）和紧密结合（组分2）的脂质成分进行研究的方法，被用于测定原核细胞的脂质组。检测到游离脂肪酸C16:0、C18:1（组分1）、C14:0、C16:0和C18:2（组分2）。与DNA结合的脂质的两个组分都以磷脂酰甘油、磷脂酰丝氨酸和溶血磷脂酰肌醇的存在为特征。醇溶性组分1还可能含有磷脂酰胆碱和磷脂酰肌醇，而组分2可能含有甘油三酯。与气相色谱法相比，ESI-LC-MS为类核脂质组的研究提供了新的可能性，能够对细菌细胞中与DNA结合的脂质进行更详细的研究。
