Vetchinkina E P, Gorshkov V Iu, Ageeva M V, Gogolev Iu V, Nikitina V E
Mikrobiologiia. 2015 Jan-Feb;84(1):78-89.
Activation of expression of the lcc4 and tir genes encoding laccase and tyrosinase was observed during transition of a xylotrophic basidiomycete Lentinus edodes from the vegetative to the generative growth stages. This was especially pronounced in the brown mycelial mat (the stage preceding formation of the fruiting bodies). Development of this structure was shown to be associated with a sharp increase of laccase and tyrosinase activities, as well as with rearrangements in the phenol oxidase complex. Formation of the tissues with thickened cell walls was associated with enhanced expression of the chi and exg1 genes encoding chitinase and glucanase, respectively. Exogenous treatment of the vegetative mycelium with laccase preparation from the brown mycelial mat promoted formation of this morphological structure. Activation of the lcc4, tir, chi, and exg1 genes may be used as a marker of readiness to fruition in xylotrophic fungi.
在木腐担子菌香菇从营养生长阶段向生殖生长阶段转变过程中，观察到编码漆酶和酪氨酸酶的lcc4和tir基因的表达被激活。这在棕色菌丝体垫（子实体形成前的阶段）中尤为明显。已表明这种结构的发育与漆酶和酪氨酸酶活性的急剧增加以及酚氧化酶复合物的重排有关。细胞壁增厚的组织的形成分别与编码几丁质酶和葡聚糖酶的chi和exg1基因的表达增强有关。用来自棕色菌丝体垫的漆酶制剂对外源营养菌丝体进行处理促进了这种形态结构的形成。lcc4、tir、chi和exg1基因的激活可作为木腐真菌准备结实的标志。
