Poroshina M N, Doronina N V, Kaparullina E N, Trotsenko Iu A
Mikrobiologiia. 2015 Jan-Feb;84(1):90-7.
A strain (PK1) of facultative methylobacteria growing on methanol as a carbon and energy source was isolated from carex rhizosphere (Pamukkale National Park, Turkey). The cells were nonmotile gram-negative rods propagating by binary fission. The organism was a strict anaerobe, oxidase- and catalase-positive. Optimal growth occurred at 29°C, pH 8.0-8.5, and 0.5% NaCl; no growth occurred at 2% NaCl. The organism used the ribulose bisphosphate pathway of C1 assimilation. Predominant fatty acids were 11-octodecenoic (18:1ω7) and cis-hexadecenoic (16:1ω7c). Phosphatidylethanolamine and diphosphatidylglycerol were the dominant phospholipids. Q8 was the main ubiquinone. DNA G+C content was 55.4 mol % (mp). Sequencing of the 16S rRNA gene revealed that strain PK1 belonged to the genus Advenella with 98.8 and 99.2% similarity to the type strains A. incenata CCUG 45225T and A. kashmirensis WT001T, respectively. DNA-DNA homology of strain PK1 and A. kashmirensis WT001T was 70%. While MALDI analysis confirmed their close clusterization, RAPD analysis revealed the differences between strain PKI and other Advenella strains. Based on its geno- and phenotypic properties, the isolate PK1 was classified as A. kashmirensis subsp. methylica PK1 (VKM-B 2850 = DSM 27514), the first known methylotroph of the genus Advenella.
从土耳其棉花堡国家公园苔草根际分离出一株兼性甲基杆菌（PK1），该菌株以甲醇作为碳源和能源生长。细胞为革兰氏阴性、无运动性的杆菌，通过二分裂繁殖。该微生物为严格厌氧菌，氧化酶和过氧化氢酶呈阳性。最适生长温度为29℃，pH值为8.0 - 8.5，NaCl浓度为0.5%；在2% NaCl条件下不生长。该微生物利用核酮糖二磷酸途径进行一碳同化。主要脂肪酸为11 - 十八碳烯酸（18:1ω7）和顺式十六碳烯酸（16:1ω7c）。磷脂酰乙醇胺和双磷脂酰甘油是主要的磷脂。泛醌Q8是主要的泛醌。DNA G + C含量为55.4 mol%（熔点法）。16S rRNA基因测序表明，菌株PK1属于Advenella属，与模式菌株A. incenata CCUG 45225T和A. kashmirensis WT001T的相似性分别为98.8%和99.2%。菌株PK1与A. kashmirensis WT001T的DNA - DNA同源性为70%。虽然基质辅助激光解吸电离分析证实了它们的紧密聚类，但随机扩增多态性DNA分析揭示了菌株PKI与其他Advenella菌株之间的差异。基于其基因型和表型特征，分离株PK1被分类为A. kashmirensis subsp. methylica PK1（VKM - B 2850 = DSM 27514），这是Advenella属中首个已知的甲基营养型菌株。
