Doronina Nina, Darmaeva Tsyregma, Trotsenko Yuri
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia.
Syst Appl Microbiol. 2003 Sep;26(3):382-9. doi: 10.1078/072320203322497419.
A new, moderately haloalkaliphilic and restricted-facultatively methylotrophic bacterium (strain Bur2T) with the ribulose monophosphate pathway of carbon assimilation is described. The isolate, which utilizes methanol, methylamine and fructose, is an aerobic, Gram-negative, asporogenous, motile short rod multiplying by binary fission. It is auxotrophic for vitamin B12, and requires NaHCO3 or NaCl for growth in alkaline medium. Cellular fatty acids profile consists primarily of straight-chain saturated C16:0, unsaturated C16:1 and C18:1 acids. The major ubiquinone is Q-8. The dominant phospholipids are phosphatidylethanolamine and phosphatidylglycerol. Diphosphatidylglycerol is also present. Optimal growth conditions are 25-29 degrees C, pH 8.5-9.0 and 2-3% (w/v) NaCl. Cells accumulate ectoine and glutamate as the main osmoprotectants. The G + C content of the DNA is 45.0 mol%. Based on 16S rDNA sequence analysis and DNA-DNA relatedness (25-35%) with type strains of marine and soda lake methylobacteria belonging to the genus Methylophaga, the novel isolate was classified as a new species of this genus and named Methylophaga natronica (VKM B-2288T).
描述了一种新的中度嗜盐碱且兼性甲基营养受限的细菌（菌株Bur2T），其具有核糖单磷酸途径的碳同化作用。该分离株利用甲醇、甲胺和果糖，是需氧的革兰氏阴性、无芽孢、运动性短杆菌，通过二分裂进行繁殖。它对维生素B12营养缺陷，在碱性培养基中生长需要NaHCO3或NaCl。细胞脂肪酸谱主要由直链饱和C16:0、不饱和C16:1和C18:1酸组成。主要的泛醌是Q-8。主要的磷脂是磷脂酰乙醇胺和磷脂酰甘油。也存在双磷脂酰甘油。最佳生长条件是25-29℃、pH 8.5-9.0和2-3%（w/v）NaCl。细胞积累ectoine和谷氨酸作为主要的渗透保护剂。DNA的G + C含量为45.0 mol%。基于16S rDNA序列分析以及与属于嗜甲基菌属的海洋和苏打湖甲基杆菌模式菌株的DNA-DNA相关性（25-35%），该新分离株被归类为该属的一个新物种，并命名为嗜甲基嗜钠菌（VKM B-2288T）。
