Wei Guijiang, Yang Fenglian, Liang Lina, Liang Limei, Wei Chuandong, Luo Bin, Wei Yesheng, Nong Legen, Tang Yujin, Wang Junli
Department of Medical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China.
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Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2015 Feb;50(2):127-30.
To evaluate the relationship between osteopontin gene genetic polymorphisms and susceptibility of nasopharyngeal carcinoma in Guangxi Zhuang people.
With a hospital based case-control study, osteopontin gene polymorphisms were compared between patients with nasopharyngeal carcinoma and healthy outpatients as a controls in Zhuang population in Guangxi. The single nucleotide polymorphisms at rs1126772 and rs9138 sites of the osteopontin gene were determined by polymerase chain reaction-single base extension technique (PCR-SBE) and DNA sequencing technology. The comparison between genotype and allele frequency distribution differences in case and control group was accomplished by a χ(2) test. The frequencies of haplotypes in osteopontin gene in different groups were analyzed.
There were no differences between the patients and controls in the genotype or allele frequencies of osteopontin gene rs1126772 site (
GA/GG: OR = 0.94, 95%CI 0.37-2.37, χ(2) = 0.182, P = 0.891; AA/GG:OR = 0.86, 95%CI 0.35-2.12, χ(2) = 0.834, P = 0.773) or rs9138 site (
CA/CC: OR = 1.42, 95%CI 0.88-2.29, χ(2) = 2.023, P = 0.155; AA/CC:OR = 1.77, 95%CI 0.78-4.01, χ(2) = 1.901, P = 0.168). The frequency of GA haplotype in the patients was significantly higher than that in the controls (P = 0.003), and the GA haplotype was associated with a significantly increased risk of nasopharyngeal carcinoma (OR = 4.84, 95%CI 1.59-14.71).
The haplotype GA of osteopontin gene rs1126772 and rs9138 sites increases the risk of nasopharyngeal carcinoma in Guangxi Zhuang people.
评估广西壮族人群骨桥蛋白基因多态性与鼻咽癌易感性之间的关系。
采用基于医院的病例对照研究，比较广西壮族人群中鼻咽癌患者与健康门诊对照者的骨桥蛋白基因多态性。通过聚合酶链反应-单碱基延伸技术（PCR-SBE）和DNA测序技术检测骨桥蛋白基因rs1126772和rs9138位点的单核苷酸多态性。采用χ²检验比较病例组和对照组基因型及等位基因频率分布差异。分析不同组中骨桥蛋白基因单倍型频率。
骨桥蛋白基因rs1126772位点（GA/GG：OR = 0.94，95%CI 0.37 - 2.37，χ² = 0.182，P = 0.891；AA/GG：OR = 0.86，95%CI 0.35 - 2.12，χ² = 0.834，P = 0.773）或rs9138位点（CA/CC：OR = 1.42，95%CI 0.88 - 2.29，χ² = 2.023，P = 0.155；AA/CC：OR = 1.77，95%CI 0.78 - 4.01，χ² = 1.901，P = 0.168）的基因型和等位基因频率在患者与对照者之间无差异。患者中GA单倍型频率显著高于对照者（P = 0.003），且GA单倍型与鼻咽癌风险显著增加相关（OR = 4.84，95%CI 1.59 - 14.71）。
骨桥蛋白基因rs1126772和rs9138位点的GA单倍型增加广西壮族人群患鼻咽癌的风险。
