一个用于研究酵母细胞中DNA复制应激的高通量共聚焦荧光显微镜平台。

A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.

作者信息

Torres Nikko P, Brown Grant W

机构信息

Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.

出版信息

Methods Mol Biol. 2015;1300:1-12. doi: 10.1007/978-1-4939-2596-4_1.

DOI:10.1007/978-1-4939-2596-4_1

PMID:25916702

Abstract

High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.

摘要

对表达荧光蛋白的酵母细胞进行高通量成像，可用于在空间组织背景下理解生物途径。在此，我们描述一种方法，使用珀金埃尔默Opera高内涵共聚焦成像显微镜，以384孔板形式对表达用绿色荧光蛋白（GFP）和/或红色荧光蛋白（RFP）标记的蛋白质的酵母细胞进行成像，无论是否经过药物处理。