通过强阴离子交换高效液相色谱结合紫外检测法测定酵母细胞中的脱氧核糖核苷三磷酸浓度。
Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
作者信息
Jia Shaodong, Marjavaara Lisette, Buckland Robert, Sharma Sushma, Chabes Andrei
机构信息
Department of Medical Biochemistry and Biophysics, Umeå University, KBC-Building, 6th Floor, Umeå, 901 87, Sweden.
出版信息
Methods Mol Biol. 2015;1300:113-21. doi: 10.1007/978-1-4939-2596-4_8.
DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.
DNA聚合酶测定法通常用于检测生物样品中的脱氧核糖核苷三磷酸（dNTPs）。为了获得更好的特异性和准确性，已经开发了高效液相色谱（HPLC）方法来分析复杂样品中的四种dNTP。在此，我们描述了一种简单的方法，该方法使用等度强阴离子交换（SAX）色谱分离结合紫外检测（UV）来分析酿酒酵母中的四种dNTP。该方法可应用于其他酵母或细菌物种。
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