高效液相色谱法分析5'-脱氧核糖核苷酸
[Analysis of 5'-deoxyribonucleotides by high-effective liquid chromatography].
作者信息
Galkina O V, Pupkova V I
出版信息
Prikl Biokhim Mikrobiol. 1980 Mar-Apr;16(2):275-80.
PMID:7384015
Abstract
The paper describes a method of analyzing 5'-deoxyribonucleotides by high-effective liquid chromatography on the strong-basic anion exchange resin Aminex A-27 in a nongradient phosphate borate buffer with ethanol addition. This method is useful for simultaneous separation of up to 6 UV-absorbing impurities--mononucleotides and nucleosides--with a sensitivity of 0.01 to 0.025 microgram.
摘要
本文描述了一种在添加乙醇的非梯度磷酸盐硼酸盐缓冲液中，采用强碱性阴离子交换树脂Aminex A - 27通过高效液相色谱法分析5'-脱氧核糖核苷酸的方法。该方法可用于同时分离多达6种具有紫外吸收的杂质——单核苷酸和核苷，灵敏度为0.01至0.025微克。
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