Phan Nhu T N, Fletcher John S, Sjövall Peter, Ewing Andrew G
Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden ; National Center Imaging Mass Spectrometry, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden ; Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, SE-50115 Borås, Sweden.
Surf Interface Anal. 2014 Nov;46(Suppl 1):123-126. doi: 10.1002/sia.5547.
(fruit fly) has a relatively simple nervous system but possesses high order brain functions similar to humans. Therefore, it has been used as a common model system in biological studies, particularly drug addiction. Here, the spatial distribution of biomolecules in the brain of the fly was studied using time-of flight secondary ion mass spectrometry (ToF-SIMS). Fly brains were analyzed frozen to prevent molecular redistribution prior to analysis. Different molecules were found to distribute differently in the tissue, particularly the eye pigments, diacylglycerides, and phospholipids, and this is expected to be driven by their biological functions in the brain. Correlations in the localization of these molecules were also observed using principal components analysis of image data, and this was used to identify peaks for further analysis. Furthermore, consecutive analyses following 10 keV Ar sputtering showed that different biomolecules respond differently to Ar sputtering. Significant changes in signal intensities between consecutive analyses were observed for high mass molecules including lipids.
果蝇拥有相对简单的神经系统，但具备与人类相似的高级脑功能。因此，它已被用作生物学研究，尤其是药物成瘾研究中的常见模型系统。在此，使用飞行时间二次离子质谱（ToF-SIMS）研究了果蝇大脑中生物分子的空间分布。在分析前将果蝇大脑冷冻以防止分子重新分布。发现不同分子在组织中的分布不同，尤其是眼色素、二酰基甘油和磷脂，预计这是由它们在大脑中的生物学功能驱动的。还使用图像数据的主成分分析观察了这些分子定位的相关性，并用于识别峰以进行进一步分析。此外，10 keV氩溅射后的连续分析表明，不同生物分子对氩溅射的反应不同。对于包括脂质在内的高质量分子，连续分析之间的信号强度出现了显著变化。
