用丙二醇冷冻的仓鼠卵母细胞进行的仓鼠卵母细胞穿透试验：与未冷冻卵母细胞的比较。

Hamster oocyte penetration tests with oocytes frozen in propanediol: comparison with non-frozen oocytes.

作者信息

Sachs H H, Pink M J, Gwatkin R B

机构信息

Reproductive and Developmental Biology, Research Institute and Gynecology, Cleveland Clinic Foundation, Ohio.

出版信息

Gamete Res. 1989 Sep;24(1):31-4. doi: 10.1002/mrd.1120240106.

DOI:10.1002/mrd.1120240106

PMID:2591850

Abstract

Hamster oocytes were frozen using a 1,2-propanediol-sucrose procedure, which resulted in over 90% survival. After thawing and zona removal the oocytes were compared with non-frozen oocytes in a zona-free hamster egg test employing spermatozoa from human semen donors and suspected infertility patients. Similar data were obtained, indicating that propanediol-sucrose frozen hamster eggs may be used in place of fresh eggs for convenience and to avoid scheduling problems.

摘要

仓鼠卵母细胞采用1,2 - 丙二醇 - 蔗糖法冷冻，其存活率超过90%。解冻并去除透明带后，在无透明带仓鼠卵试验中，将这些卵母细胞与未冷冻的卵母细胞进行比较，该试验使用来自人类精液捐献者和疑似不育患者的精子。获得了相似的数据，这表明丙二醇 - 蔗糖冷冻的仓鼠卵可代替新鲜卵使用，以方便操作并避免时间安排问题。