Onoja Samuel Okwudili, Madubuike Gideon Kelechi, Ezeja Maxwell Ikechukwu
J Basic Clin Physiol Pharmacol. 2015 Sep;26(5):465-70. doi: 10.1515/jbcpp-2014-0087.
Daniella oliveri is a deciduous plant that is commonly found in savanna and open grassland. Various parts of the plant is used by herbalist in the management of different ailments. The present study aims at investigating the hepatoprotective and antioxidant activity of the methanolic extract of D. oliveri leaves.
The hepatoprotective activity was investigated using carbon tetrachloride (CCl4)-induced hepatotoxicity in albino rats. The antioxidant activity was determined using both in vitro (2, 2-diphenyl-1-picrylhydrazine photometric assay) and in vivo (malondialdehyde and catalase level assay) models.
The pretreatment with extract (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) produced a significant (p<0.05) dose-dependent increase in hepatoprotective activity when compared with the negative control group. The extract (25-400 μg/mL concentration) produced a concentration-dependent increase in antioxidant activity in 2, 2-diphenyl-1-picrylhydrazine (DPPH) photometric assay. The IC50 of the extract in DPPH photometric assay was 400 μg/mL concentrations. The extract and silymarin showed a significant (p<0.05) dose-dependent increase in catalase level in treated rats when compared with the negative control group. Also, the extract and silymarin produced a significant (p<0.05) dose-dependent decrease in malondialdehyde level in treated rats when compared with the negative control group.
The results of the study suggest that D. oliveri leaves has a potent hepatoprotective activity that may be linked to its antioxidant activities and validates its use in the traditional management of liver disorders.
奥利弗氏丹妮艾拉树是一种落叶植物，常见于热带稀树草原和开阔草原。草药医生会使用该植物的各个部位来治疗不同疾病。本研究旨在调查奥利弗氏丹妮艾拉树叶甲醇提取物的肝保护和抗氧化活性。
采用四氯化碳（CCl4）诱导白化大鼠肝毒性来研究肝保护活性。使用体外（2,2-二苯基-1-苦基肼光度法）和体内（丙二醛和过氧化氢酶水平测定）模型来测定抗氧化活性。
与阴性对照组相比，提取物（100、200和400mg/kg）和水飞蓟宾（100mg/kg）预处理后肝保护活性呈显著（p<0.05）剂量依赖性增加。在2,2-二苯基-1-苦基肼（DPPH）光度法中，提取物（浓度为25-400μg/mL）的抗氧化活性呈浓度依赖性增加。提取物在DPPH光度法中的IC50为400μg/mL浓度。与阴性对照组相比，提取物和水飞蓟宾使治疗大鼠的过氧化氢酶水平呈显著（p<0.05）剂量依赖性增加。此外，与阴性对照组相比，提取物和水飞蓟宾使治疗大鼠的丙二醛水平呈显著（p<0.05）剂量依赖性降低。
研究结果表明，奥利弗氏丹妮艾拉树叶具有强大的肝保护活性，这可能与其抗氧化活性有关，并证实了其在传统肝病治疗中的应用价值。
