Onoja Samuel O, Ihejirika Ginika Q, Nwankudu Oluchi N, Omeh Yusuf N, Ezeja Maxwell I
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Michael Okpara University of Agriculture, PMB 7267, Umudike, Abia State, Nigeria.
Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, PMB 7267, Umudike, Abia State, Nigeria.
J Pharm (Cairo). 2018 May 29;2018:6810620. doi: 10.1155/2018/6810620. eCollection 2018.
belongs to the family Crassulaceae and it is commonly used in the ethnomedical practices. This study investigated the antidiarrheal and antioxidant properties of methanol extract of leaf harvested from South-Eastern Nigeria in mice. Cold maceration method in 80% methanol was adopted in the extract preparation. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays were used to evaluate the antioxidant property while castor oil-induced diarrhea, small intestinal transit, and enteropooling models were used for the antidiarrheal investigation. The effects of the extract (50, 100, and 200 mg/kg) were compared to distilled water (10 ml/kg) and loperamide (5 mg/kg). The extract produced concentration dependent increase in antioxidant effect in both DPPH and FRAP assay. The extract caused a significant ( < 0.05) reduction in mean stool output, percentage of wet stools, small intestinal transit, and intestinal fluid accumulation in the treated mice when compared to the distilled water treated mice. The study validates the use of in the ethnomedical management of diarrhea.
属于景天科，常用于民族医学实践。本研究调查了从尼日利亚东南部采集的叶片甲醇提取物对小鼠的止泻和抗氧化特性。提取物制备采用80%甲醇冷浸法。采用2,2-二苯基-1-苦基肼（DPPH）和铁还原抗氧化能力（FRAP）测定法评估抗氧化特性，同时采用蓖麻油诱导的腹泻、小肠转运和肠积液模型进行止泻研究。将提取物（50、100和200mg/kg）的效果与蒸馏水（10ml/kg）和洛哌丁胺（5mg/kg）进行比较。提取物在DPPH和FRAP测定中均产生了浓度依赖性的抗氧化作用增强。与蒸馏水处理的小鼠相比，提取物使处理小鼠的平均粪便产量、湿粪百分比、小肠转运和肠液积聚显著（<0.05）降低。该研究验证了其在腹泻民族医学管理中的应用。
