Ceni Giovana, da Silva Patrícia Costa, Lerin Lindomar, Charin Rafael Mengotti, Oliveira J Vladimir, Toniazzo Geciane, Treichel Helen, Oestreicher Enrique Guillermo, de Oliveira Débora
Universidade Federal do Rio de Janeiro, Instituto de Química, Cidade Universitária, 21941-909 Rio de Janeiro, RJ, Brazil; Universidade Regional Integrada do Alto Uruguai e das Missões - URI - Campus de Erechim, Departamento de Engenharia de Alimentos, Av. 7 de Setembro, 1621, 99700-000 Erechim, RS, Brazil.
Universidade Regional Integrada do Alto Uruguai e das Missões - URI - Campus de Erechim, Departamento de Engenharia de Alimentos, Av. 7 de Setembro, 1621, 99700-000 Erechim, RS, Brazil.
Enzyme Microb Technol. 2010 May 5;46(6):513-9. doi: 10.1016/j.enzmictec.2010.01.007. Epub 2010 Feb 1.
The aim of this work is to report the enzymatic transesterification production of 1-glyceryl benzoate in compressed n-butane, using a commercial immobilized lipase, Novozym 435. For this purpose, reaction experiments were performed on the basis of phase equilibrium data of the system methyl benzoate/n-butane, measured using the static synthetic method with a variable-volume view cell in the temperature range of 313.15-343.15K and pressures up to 12MPa, in the entire compositional range of n-butane. Results indicate the existence of a relatively complex phase behavior for all temperatures investigated with the occurrence of vapor-liquid and liquid-liquid phase transitions. Reaction results showed that the strategy adopted for the experimental design proved to be useful in optimizing the reaction conversion in pressurized n-butane and Novozym 435. The optimum conditions were found to be 5.5wt% of enzyme, methyl benzoate to glycerol molar ratio of 3:1, 50°C and 6h of reaction, affording about 6% of 1-glyceryl benzoate yield.
本工作的目的是报道使用商业固定化脂肪酶诺维信435在压缩正丁烷中通过酶促酯交换反应生产苯甲酸单甘油酯。为此，基于苯甲酸甲酯/正丁烷体系的相平衡数据进行反应实验，该相平衡数据是在313.15 - 343.15K温度范围和高达12MPa压力下，使用变体积视窗池的静态合成法在正丁烷的整个组成范围内测量得到的。结果表明，在所研究的所有温度下均存在相对复杂的相行为，出现了气 - 液和液 - 液相变。反应结果表明，所采用的实验设计策略被证明有助于优化在加压正丁烷和诺维信435中的反应转化率。发现最佳条件为酶含量5.5wt%、苯甲酸甲酯与甘油摩尔比3:1、50°C和反应6小时，苯甲酸单甘油酯产率约为6%。
