Zheng Si-Hao, Li Ya-Kang, Ren Wei-Guang, Huang Lin-Fang
Yao Xue Xue Bao. 2014 Dec;49(12):1730-8.
To establish a new method for identifying genus of Lilium by DNA barcoding technology, ITS, ITS2, psbA-trnH, matK and rbcL sequences were analyzed in term of variation of inter- and intra-species, barcoding gap, neighbor-joining tree to distinguish genus of Lilium based on 978 sequences from experimental and GenBank database, and identification efficiency was evaluated by Nearest distance and BLAST1 methods. The results showed that DNA barcoding could identify different species in genus of Lilium. ITS sequence performed higher identification efficiency, and had significant difference between intra- and inter-species. And NJ tree could also divide species into different clades. Results indicate that DNA barcoding can identify genus of Lilium accurately. ITS sequence can be the optimal barcode to identify species of Lilium.
为建立一种利用DNA条形码技术鉴定百合属植物的新方法，基于来自实验和GenBank数据库的978条序列，从种间和种内变异、条形码间隙、邻接树等方面分析了ITS、ITS2、psbA-trnH、matK和rbcL序列，以区分百合属植物，并通过最近距离法和BLAST1法评估鉴定效率。结果表明，DNA条形码可鉴定百合属不同物种。ITS序列的鉴定效率较高，种内和种间存在显著差异。NJ树也可将物种分为不同分支。结果表明，DNA条形码能够准确鉴定百合属植物。ITS序列可作为鉴定百合属物种的最佳条形码。
