Zhang Peng, Han Guohua, Gao Pei, Qiao Kun, Ren Yusheng, Liang Chun, Leng Bing, Wu Zonggui
Nat Prod Commun. 2015 Feb;10(2):263-6.
For this study, peripheral blood samples were collected from human volunteers. Mononuclear cells (MNC) were separated by density centrifugation and were induced to differentiate into endothelial progenitor cells (EPCs) in vitro. Different concentrations of rapamycin and silymarin were introduced to the EPCs over 24 hours and then EPCs were analyzed for proliferation, migration, apoptosis and angiogenesis. Compared with the control group, rapamycin (1, 10, 100 ng/mL) inhibited the proliferation and migration of EPCs in a concentration dependent manner (P<0.05). Silymarin (50, 100 μg/mL) enhanced the proliferation and migration of EPCs and inhibited apoptosis in a concentration dependent manner (P<0.05). By adding rapamycin (1 ng/mL) and silymarin (25, 50, 100 μg/mL) over 24 hours, silymarin inhibited the pro-apoptotic effect of rapamycin on EPCs, and reversed the inhibition of proliferation, migration and angiogenesis of EPCs by rapamycin (P<0.05).
在本研究中，从人类志愿者身上采集外周血样本。通过密度离心分离单核细胞（MNC），并在体外诱导其分化为内皮祖细胞（EPC）。在24小时内将不同浓度的雷帕霉素和水飞蓟宾引入EPC，然后分析EPC的增殖、迁移、凋亡和血管生成情况。与对照组相比，雷帕霉素（1、10、100 ng/mL）以浓度依赖性方式抑制EPC的增殖和迁移（P<0.05）。水飞蓟宾（50、100 μg/mL）以浓度依赖性方式增强EPC的增殖和迁移并抑制凋亡（P<0.05）。在24小时内加入雷帕霉素（1 ng/mL）和水飞蓟宾（25、50、100 μg/mL），水飞蓟宾抑制了雷帕霉素对EPC的促凋亡作用，并逆转了雷帕霉素对EPC增殖、迁移和血管生成的抑制作用（P<0.05）。
