Almasi Ehsan, Gharagozloo Marjan, Eskandari Nahid, Almasi Afshin, Sabzghabaee Ali Mohammad
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Clinical Research Development Unit, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Iran J Allergy Asthma Immunol. 2017 Apr;16(2):107-119.
Silybum marianum, is known to have anti-inflammatory, hepatoprotective and anticarciogenic effects. The aim of this study was to compare effects of Silymarin, Rapamycin and FK506 on proliferation and apoptosis of human T cells stimulated with Con A. Peripheral blood mononuclear cells (PBMC) were stimulated with concavalin A (Con A) (5µg/mL) and then treated with different inhibitors (silymarin, rapamycin and FK506) in various concentrations (5 days). Cells were examined using carboxyfluorescein succinimidyl ester (CFSE) assay for proliferation. Then cell apoptosis was analyzed by FITC annexin V/PI staining and flow cytometry. The effects of drugs on the activation of poly ADP ribose polymerase (PARP) pathway in PBMCs stimulated with Con A and treated with IC50 dose of drugs for 5 days were evaluated using the PathScan cleaved PARP sandwich ELISA kit. The results indicated that silymarin inhibited T cell proliferation. In addition, our results pointed out that 100 μM and 200 μM of silymarin significantly have more inhibitory effect on T cells proliferation than FK506 and rapamycin. None of these drugs at IC50 concentration had affected the level of cleaved PARP. Overall, with superior efficacy and lesser toxicity in comparison with other immunosuppressive drugs, silymarin could be a suitable choice of therapy for certain diseases.
水飞蓟，已知具有抗炎、保肝和抗癌作用。本研究的目的是比较水飞蓟素、雷帕霉素和FK506对伴刀豆球蛋白A刺激的人T细胞增殖和凋亡的影响。外周血单个核细胞（PBMC）用刀豆球蛋白A（Con A）（5μg/mL）刺激，然后用不同浓度的抑制剂（水飞蓟素、雷帕霉素和FK506）处理（5天）。使用羧基荧光素琥珀酰亚胺酯（CFSE）测定法检测细胞增殖。然后通过FITC膜联蛋白V/PI染色和流式细胞术分析细胞凋亡。使用PathScan裂解PARP夹心ELISA试剂盒评估药物对用Con A刺激并用IC50剂量的药物处理5天的PBMC中多聚ADP核糖聚合酶（PARP）途径激活的影响。结果表明，水飞蓟素抑制T细胞增殖。此外，我们的结果指出，100μM和200μM的水飞蓟素对T细胞增殖的抑制作用明显大于FK506和雷帕霉素。这些药物在IC50浓度下均未影响裂解PARP的水平。总体而言，与其他免疫抑制药物相比，水飞蓟素具有更高的疗效和更低的毒性，可能是某些疾病的合适治疗选择。
