Okano Junko, Kojima Hideto, Katagi Miwako, Nakae Yuki, Terashima Tomoya, Nakagawa Takahiko, Kurakane Takeshi, Okamoto Naoki, Morohashi Keita, Maegawa Hiroshi, Udagawa Jun
Division of Anatomy and Cell Biology, Shiga University of Medical Science, Shiga, Japan.
Department of Stem Cell Biology and Regenerative Medicine, Shiga University of Medical Science, Shiga, Japan.
Biochem Biophys Res Commun. 2015 Jun 12;461(4):695-701. doi: 10.1016/j.bbrc.2015.04.094. Epub 2015 Apr 25.
Bone marrow-derived cells (BMDCs) can migrate into the various organs in the mice irradiated by ionizing radiation (IR). However, it may not be the case in the skin. While IR is used for bone marrow (BM) transplantation, studying with the epidermal sheets demonstrated that the BMDC recruitment is extraordinarily rare in epidermis in the mouse. Herein, using the chimera mice with BM from green fluorescent protein (GFP) transgenic mice, we simply examined if BMDCs migrate into any layers in the total skin, as opposed to the epidermal sheets, in response to IR. Interestingly, we identified the presence of GFP-positive (GFP(+)) cells in the epidermis-dermis junction in the total skin sections although the epidermal cell sheets failed to have any GFP cells. To examine a possibility that the cells in the junction could be mechanically dissociated during separating epidermal sheets, we then salvaged such dissociated cells and examined its characteristics. Surprisingly, some GFP(+) cells were found in the salvaged cells, indicating that these cells could be derived from BM. In addition, such BMDCs were also associated with inflammation in the junction. In conclusion, BMDCs can migrate to and reside in the epidermis-dermis junction after IR.
骨髓来源的细胞（BMDCs）能够迁移到受电离辐射（IR）照射的小鼠的各个器官中。然而，在皮肤中情况可能并非如此。当IR用于骨髓（BM）移植时，对表皮片的研究表明，在小鼠的表皮中BMDC的募集极其罕见。在此，我们使用来自绿色荧光蛋白（GFP）转基因小鼠的骨髓构建嵌合小鼠，简单地检测了BMDCs是否会响应IR迁移到全层皮肤的任何层中，而不是像表皮片那样。有趣的是，我们在全层皮肤切片的表皮 - 真皮交界处发现了GFP阳性（GFP(+)）细胞，尽管表皮细胞片层中没有任何GFP细胞。为了研究在分离表皮片层过程中交界处的细胞是否可能被机械解离，我们随后挽救了这些解离的细胞并检测其特性。令人惊讶的是，在挽救的细胞中发现了一些GFP(+)细胞，表明这些细胞可能来源于骨髓。此外，这种BMDCs也与交界处的炎症有关。总之，IR后BMDCs能够迁移到表皮 - 真皮交界处并驻留在那里。
