社区居住的美国黑人和白人的24,25 - 二羟基维生素D3与维生素D状态
24,25-Dihydroxyvitamin d3 and vitamin D status of community-dwelling black and white Americans.
作者信息
Berg Anders H, Powe Camille E, Evans Michele K, Wenger Julia, Ortiz Guillermo, Zonderman Alan B, Suntharalingam Pirianthini, Lucchesi Kathryn, Powe Neil R, Karumanchi S Ananth, Thadhani Ravi I
机构信息
Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA;
Division of Endocrinology, Massachusetts General Hospital, Boston, MA;
出版信息
Clin Chem. 2015 Jun;61(6):877-84. doi: 10.1373/clinchem.2015.240051. Epub 2015 Apr 28.
BACKGROUND
24,25-Dihydroxyvitamin D [24,25(OH)2D] is a metabolite of 25-hydroxyvitamin D (25D). Blacks frequently have low total 25D without manifestations of vitamin D deficiency, suggesting that total serum 25D may incorrectly reflect vitamin D status in different racial groups. The ratio of serum 24,25(OH)2D to 25D [vitamin D metabolite ratio (VMR)] represents a new candidate biomarker for vitamin D status.
METHODS
We measured 24,25(OH)2D3 and 25D3 by mass spectrometry in a random community cohort of black (n = 212) and white (n = 164) Americans to evaluate VMR as a marker for vitamin D status. We measured parathyroid hormone concentrations by immunoassay to compare VMR and 25D3 against a physiological indicator of vitamin D deficiency.
RESULTS
Serum 24,25(OH)2D3 strongly correlated with 25D3 in both black and white study participants (r = 0.90, P < 0.001 and r = 0.86, P < 0.001 respectively). Blacks had lower mean 25D3 than whites [17.0 (7.8) vs 27.5 (11.3) ng/mL; 42.4 (19.5) vs 68.6 (28.2) nmol/L, P < 0.001] and lower mean 24,25(OH)2D3 [2.1 (1.3) vs 3.6 (2.0) ng/mL; 5.1 (3.1) vs 8.7 (4.8) nmol/L, P < 0.001]. In contrast to total 25D3 concentrations, mean VMR values were similar in blacks and whites [11.9 (4.0) vs 12.5 (3.4), P = 0.16, respectively] and were negatively correlated with parathyroid hormone concentrations in both races (rs = -0.26, P < 0.001, and rs = -0.25, P < 0.001, respectively).
CONCLUSIONS
Our results provide further evidence that measurement of total 25D for assessment of vitamin D status in patients of African descent deserves reevaluation and suggest that alternative measures such as VMR should be considered.
背景
24,25 - 二羟基维生素D [24,25(OH)₂D] 是25 - 羟基维生素D（25D）的一种代谢产物。黑人的总25D水平常常较低，但并无维生素D缺乏的表现，这表明血清总25D可能无法准确反映不同种族人群的维生素D状态。血清24,25(OH)₂D与25D的比值 [维生素D代谢产物比值（VMR）] 是一种新的维生素D状态候选生物标志物。
方法
我们通过质谱法对一个由212名黑人及164名白人组成的随机社区队列中的24,25(OH)₂D₃和25D₃进行了测量，以评估VMR作为维生素D状态标志物的情况。我们通过免疫测定法测量了甲状旁腺激素浓度，以便将VMR和25D₃与维生素D缺乏的生理指标进行比较。
结果
在黑人和白人研究参与者中，血清24,25(OH)₂D₃与25D₃均呈强相关（分别为r = 0.90，P < 0.001和r = 0.86，P < 0.001）。黑人的平均25D₃水平低于白人 [17.0（7.8）对27.5（11.3）ng/mL；42.4（19.5）对68.6（28.2）nmol/L, P < 0.001]，且平均24,25(OH)₂D₃水平也较低 [2.1（1.3）对3.6（2.0）ng/mL；5.1（3.1）对8.7（4.8）nmol/L, P < 0.001]。与总25D₃浓度不同，黑人和白人的平均VMR值相似 [分别为11.9（4.0）对12.5（3.4），P = 0.16]，且在两个种族中均与甲状旁腺激素浓度呈负相关（分别为rs = -0.26，P < 0.001和rs = -0.25，P < 0.001）。
结论
我们的结果进一步证明，在评估非洲裔患者的维生素D状态时，测量总25D值得重新评估，并表明应考虑诸如VMR等替代测量方法。
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