Hagen S R, Frost B, Augustin J
University of Idaho, Department of Bacteriology and Biochemistry, Moscow, ID 83843.
J Assoc Off Anal Chem. 1989 Nov-Dec;72(6):912-6.
A precolumn phenylisothiocyanate derivatization method is described for the determination of amino acids in protein hydrolysates from a wide variety of complex food matrixes, with and without performic acid oxidation pretreatment. Analysis of samples that were not pretreated with performic acid was necessary since this pretreatment destroyed an average of 25% of the histidine and 87% of the tyrosine present in the food samples. This method is rapid and reproducible; coefficients of variation between duplicate analyses of the same food item were less than 5% for a majority of the amino acids. Occasionally, variation between duplicate analyses for histidine and tyrosine was greater than 10%. Recoveries of amino acids added to samples were in the 100% range.
本文描述了一种柱前异硫氰酸苯酯衍生化方法，用于测定来自各种复杂食物基质的蛋白质水解物中的氨基酸，该方法可选择进行或不进行过甲酸氧化预处理。对未用过甲酸预处理的样品进行分析是必要的，因为这种预处理平均会破坏食物样品中25%的组氨酸和87%的酪氨酸。该方法快速且可重复；对于大多数氨基酸，同一种食物样品重复分析之间的变异系数小于5%。偶尔，组氨酸和酪氨酸重复分析之间的变异大于10%。添加到样品中的氨基酸回收率在100%左右。
