Marchiori Maiara Sulzbach, Bourckhardt Larissa E H, Lima Jessica L F, Comin Antony, Zamboni Taeline, Boiago Marcel M, Paiano Diovani, Da Silva Aleksandro S
Graduate Program in Animal Science - Universidade do Estado de Santa Catarina (UDESC), Chapecó, Santa Catarina, Brazil.
Department of Animal Science - UDESC, Chapecó, Brazil.
Trop Anim Health Prod. 2025 Jul 14;57(6):298. doi: 10.1007/s11250-025-04546-w.
This study aimed to evaluate the effects of including a blend of acid and alkaline proteases in broiler diets on performance parameters, nutrient digestibility, and intestinal morphology. A total of 180 chicks were divided into the following groups: Positive Control (PC), Negative Control (with 8% reduction in crude protein-NC), and NC + blend proteases (BP) at 125 g/t (BP125). On days 1 and 21, the broilers and the feed were weighed for performance analysis, and a digestibility test was performed from days 17 to 21. The highest body weight and weight gain were observed in the broilers of the PC and BP125 groups (P = 0.001). The addition of protease to the diet (BP125) increased the ADC of crude protein, which was statistically similar to the PC group; both were higher than those observed in the NC group. We observed that the BP125 group had more excellent digestibility of the amino acids of serine (P = 0.032), threonine (P = 0.050), valine (P = 0.042), methionine (P = 0.050), cysteine (P = 0.012), arginine (P < 0.001) and tryptophan (P = 0.05) when compared to NC; but similar to PC group. It was also possible to observe a better villus and crypt ratio when protease was used in a low-protein diet (P = 0.05). These findings suggest that protease supplementation improves nutrient utilization and performance in low-protein diets.
本研究旨在评估在肉鸡日粮中添加酸性和碱性蛋白酶混合物对生产性能参数、养分消化率和肠道形态的影响。总共180只雏鸡被分为以下几组：正对照组（PC）、负对照组（粗蛋白含量降低8% - NC）和添加125 g/t蛋白酶混合物（BP）的NC组（BP125）。在第1天和第21天，对肉鸡和饲料进行称重以进行生产性能分析，并在第17天至第21天进行消化率测试。在PC组和BP125组的肉鸡中观察到最高体重和体重增加（P = 0.001）。日粮中添加蛋白酶（BP125）提高了粗蛋白的表观消化率系数（ADC），在统计学上与PC组相似；两者均高于NC组。我们观察到，与NC组相比，BP125组的丝氨酸（P = 0.032）、苏氨酸（P = 0.050）、缬氨酸（P = 0.042）、蛋氨酸（P = 0.050）、半胱氨酸（P = 0.012）、精氨酸（P < 0.001）和色氨酸（P = 0.05）氨基酸消化率更高；但与PC组相似。当在低蛋白日粮中使用蛋白酶时，还可以观察到更好的绒毛与隐窝比例（P = 0.05）。这些发现表明，补充蛋白酶可提高低蛋白日粮中的养分利用率和生产性能。
