Rech Fabricio Valandro, Fagundes Anna Luiza Negrini, Simões Ricardo Santos, Florencio-Silva Rinaldo, Sasso Gisela Rodrigues da Silva, Taha Murched Omar, Fagundes Djalma José
EPM, UNIFESP, Brazil.
UNIFESP, Sao Paulo, SP, Brazil.
Acta Cir Bras. 2015 Apr;30(4):235-41. doi: 10.1590/S0102-865020150040000001. Epub 2015 Apr 1.
To evaluate the morphology, necrotic area and collagen content in skin flaps of rats subjected to hyperbaric oxygenation (HBO).
Forty adult rats were divided into four groups: GEC - epilated; GE/HBO - epilated subjected to HBO; GER - epilated submitted to skin flap (2 cm in width /8 cm length in the dorsal area) and GER/HBO - epilated, subjected to skin flap and HBO. HBO (2.4 ATA) was performed for two hours during seven consecutive days. In the eighth day, the rats were anesthetized and the skin flaps were removed and separated into three portions, relative to pedicle fixation. The material fixed in 10% formalin was processed for paraffin embedding; sections were stained by H.E and subjected to picrosirius-red method. The slides examined under light microscopy for evaluation of the collagen content in polarized light microscope and ImageLab(r) software (Bio-Rad).
The data showed larger area of necrosis and lower levels of collagen in the three regions of the GER group, whereas in the GER/HBO group the collagen content was similar to the GEC and GE/HBO groups.
Hyperbaric oxygenation reduced the area of necrosis and preserved the morphology and collagen content in skin flaps of rats.
评估接受高压氧治疗（HBO）的大鼠皮瓣的形态、坏死面积和胶原蛋白含量。
将40只成年大鼠分为四组：GEC组——脱毛；GE/HBO组——脱毛并接受HBO治疗；GER组——脱毛并制作皮瓣（背部区域宽2 cm/长8 cm）；GER/HBO组——脱毛、制作皮瓣并接受HBO治疗。连续7天每天进行2小时的HBO治疗（2.4 ATA）。在第8天，将大鼠麻醉，取下皮瓣并相对于蒂固定分成三部分。将固定在10%福尔马林中的材料进行石蜡包埋；切片用苏木精-伊红染色，并采用天狼星红苦味酸染色法。在光学显微镜下检查玻片，以在偏振光显微镜和ImageLab®软件（伯乐公司）下评估胶原蛋白含量。
数据显示，GER组三个区域的坏死面积更大，胶原蛋白水平更低，而GER/HBO组的胶原蛋白含量与GEC组和GE/HBO组相似。
高压氧治疗减少了大鼠皮瓣的坏死面积，保留了其形态和胶原蛋白含量。
