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从产链黑菌素的絮状链霉菌中纯化及鉴定S-腺苷高半胱氨酸脱氨酶

Purification and characterization of S-adenosylhomocysteine deaminase from streptonigrin-producing Streptomyces flocculus.

作者信息

Zulty J J, Speedie M K

机构信息

Department of Biomedicinal Chemistry, School of Pharmacy, University of Maryland, Baltimore 21201.

出版信息

J Bacteriol. 1989 Dec;171(12):6840-4. doi: 10.1128/jb.171.12.6840-6844.1989.

DOI:10.1128/jb.171.12.6840-6844.1989
PMID:2592350
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210584/
Abstract

An S-adenosylhomocysteine deaminase has been isolated and purified from streptonigrin-producing Streptomyces flocculus ATCC 13257. Deamination represents the major metabolic route of S-adenosylhomocysteine in this organism. The protein was found to be monomeric with a molecular weight of 56,100 +/- 1,600. The activity was optimal at pH 7.0 and 37 degrees C, and the deaminase was inactivated by p-chloromercuribenzoate but not by metal chelators. The Km for S-adenosylhomocysteine is 2.5 mM, and the Ki for inhibition by deoxycoformycin is 1.6 nM.

摘要

已从产生链黑菌素的弗氏链霉菌ATCC 13257中分离并纯化出一种S-腺苷同型半胱氨酸脱氨酶。脱氨作用是该生物体中S-腺苷同型半胱氨酸的主要代谢途径。发现该蛋白质为单体，分子量为56,100±1,600。其活性在pH 7.0和37℃时最佳，对氯汞苯甲酸可使该脱氨酶失活，但金属螯合剂不能使其失活。S-腺苷同型半胱氨酸的米氏常数为2.5 mM，脱氧助间型霉素抑制作用的抑制常数为1.6 nM。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f780/210584/dcb2a5f3bce8/jbacter00178-0457-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f780/210584/dcb2a5f3bce8/jbacter00178-0457-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f780/210584/dcb2a5f3bce8/jbacter00178-0457-a.jpg

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本文引用的文献

1
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Antibiot Annu. 1959;7:950-3.
2
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.一种测定酶沉降行为的方法：应用于蛋白质混合物
J Biol Chem. 1961 May;236:1372-9.
3
Pharmacological and biochemical aspects of S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase.S-腺苷同型半胱氨酸及S-腺苷同型半胱氨酸水解酶的药理学与生物化学特性
S-肌苷-L-高半胱氨酸水解酶，一种参与S-腺苷-L-甲硫氨酸循环的新型酶。
J Bacteriol. 2015 Jul;197(14):2284-91. doi: 10.1128/JB.00080-15. Epub 2015 Apr 27.
4
Identification of a 5'-deoxyadenosine deaminase in Methanocaldococcus jannaschii and its possible role in recycling the radical S-adenosylmethionine enzyme reaction product 5'-deoxyadenosine.鉴定詹氏甲烷球菌中的 5'-脱氧腺苷脱氨酶及其在循环利用自由基 S-腺苷甲硫氨酸酶反应产物 5'-脱氧腺苷中的可能作用。
J Bacteriol. 2014 Mar;196(5):1064-72. doi: 10.1128/JB.01308-13. Epub 2013 Dec 27.
5
Identification of a new class of adenosine deaminase from Helicobacter pylori with homologs among diverse taxa.从不同分类群中鉴定出幽门螺杆菌中的新型腺苷脱氨酶。
J Bacteriol. 2013 Sep;195(18):4154-60. doi: 10.1128/JB.00587-13. Epub 2013 Jul 12.
Pharmacol Rev. 1982 Sep;34(3):223-53.
4
A tryptophan C-methyltransferase involved in streptonigrin biosynthesis in Streptomyces flocculus.一种参与絮状链霉菌中链黑菌素生物合成的色氨酸C-甲基转移酶。
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5
Occurrence of S-adenosylhomocysteine hydrolase in prokaryote cells. Characterization of the enzyme from Alcaligenes faecalis and role of the enzyme in the activated methyl cycle.原核细胞中S-腺苷同型半胱氨酸水解酶的存在。粪产碱杆菌中该酶的特性及其在活性甲基循环中的作用。
Eur J Biochem. 1984 Jun 1;141(2):385-92. doi: 10.1111/j.1432-1033.1984.tb08203.x.
6
Inhibitors of adenosine deaminase.腺苷脱氨酶抑制剂
Pharmacol Ther. 1982;17(3):399-429. doi: 10.1016/0163-7258(82)90023-7.
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8
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9
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10
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